Influence of Surface Properties of New Biomaterials for Catheters on Bacterial Adhesion in Urine

导管用新型生物材料表面特性对尿液中细菌粘附的影响

• 批准号: EP/P00301X/1
• 负责人: Qi Zhao
• 金额: $ 63.88万
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FP00301X%2F1
• 关键词: Influence; Surface; Properties; New; Biomaterials

Catheter-associated urinary tract infection (CAUTIs) and encrustation have a severe impact on human health and health care costs. To date only a few types of antimicrobial coated catheters have come into clinical use, including a nitrofural-impregnated catheter, a silver alloy-hydrogel coated catheter, and a PTFE-coated catheter. Clinical trials show that these coated catheters give only an insignificant reduction in CAUTIs or encrustation formation. It is well known that both TiO2 and Ag nanoparticles exhibit bactericidal properties. However Ag nanoparticles with diameters less than 200 nm tend to aggregate spontaneously, and their stability in air, water or sunlight is not good enough for long-term application, which decreases their antibacterial performance. To solve these problems, a range of different Ag-TiO2 composite nanoparticles have been developed without aggregation. It has been demonstrated that both core-shell Ag@TiO2 nanoparticles (Ag nanoparticles coated with a thin shell of TiO2) and spherical Ag/TiO2 nanoparticles (TiO2 nanoparticles coated with Ag nanoparticles) exhibit synergistic bactericidal activities with long lasting action period in both light and dark conditions, compared with Ag and TiO2 nanoparticles. The Ag nanoparticles in Ag@TiO2 or Ag/TiO2 nanocomposites enable to active visible light excitation of TiO2, and can highly improve photocatalytic inactivation of bacteria. In this proposal two different types of Ag@TiO2-PTFE coated and Ag/TiO2-PTFE coated catheters will be developed by a so-gel technique. The two new types of coatings combine the excellent antibacterial properties of Ag@TiO2 and Ag/TiO2 nanoparticles and the non-stick properties of PTFE nanoparticles. The interactions between selected bacteria and the coated catheters in urine will be calculated and measured in order to find out optimum surface conditions of the coatings. The anti-bacterial and anti-encrustation performance of the coated full-length catheters in urine will be evaluated using bladder models that mimic in vivo environments of the human bladder for a catheter, and compared with existing silver-hydrogel coated catheter, PTFE coated catheter and uncoated silicone catheter. The cytotoxicity assays of the coated catheters will also be performed. After the project we will collaborate with local hospitals and a catheter manufacture to undertake clinical trials.

导管相关性尿路感染（CAUTI）和结垢对人类健康和医疗保健费用产生严重影响。迄今为止，只有少数类型的抗菌涂层导管进入临床使用，包括呋喃西林浸渍导管、银合金水凝胶涂层导管和PTFE涂层导管。临床试验表明，这些涂层导管只能微不足道地减少 CAUTI 或结痂的形成。众所周知，TiO2 和 Ag 纳米颗粒都具有杀菌特性。然而，直径小于200 nm的银纳米粒子容易自发聚集，其在空气、水或阳光中的稳定性不够好，不适合长期使用，从而降低了其抗菌性能。为了解决这些问题，开发了一系列不同的无聚集的 Ag-TiO2 复合纳米颗粒。研究表明，核壳型Ag@TiO2纳米颗粒（涂有一层薄薄的TiO2壳的Ag纳米颗粒）和球形Ag/TiO2纳米颗粒（涂有Ag纳米颗粒的TiO2纳米颗粒）在两种光下均表现出协同杀菌活性，且作用时间持久。和黑暗条件下，与 Ag 和 TiO2 纳米粒子相比。 Ag@TiO2或Ag/TiO2纳米复合材料中的Ag纳米粒子能够激活TiO2的可见光激发，并且可以显着提高细菌的光催化灭活效果。在该提案中，将通过 so-gel 技术开发两种不同类型的 Ag@TiO2-PTFE 涂层和 Ag/TiO2-PTFE 涂层导管。这两种新型涂层结合了Ag@TiO2和Ag/TiO2纳米颗粒的优异抗菌性能以及PTFE纳米颗粒的不粘性能。将计算和测量选定的细菌与尿液中涂层导管之间的相互作用，以找出涂层的最佳表面条件。采用模仿人体膀胱体内环境的膀胱模型来评估涂层全长导管在尿液中的抗菌和抗结垢性能，并与现有的银水凝胶涂层导管、PTFE涂层导管进行比较和无涂层硅胶导管。还将进行涂层导管的细胞毒性测定。项目结束后我们将与当地医院和导管制造商合作进行临床试验。

项目成果

Antibacterial AgNPs-PAAm-CS-PVP nanocomposite hydrogel coating for urinary catheters

• DOI: 10.1016/j.eurpolymj.2023.112260
• 发表时间: 2023-09
• 期刊: European Polymer Journal
• 影响因子: 6
• 作者: Yongwei Cai;Han Yang;Jianxiang Li;R. Gu;Yuhang Dong;Qi Zhao;Yao-Cyong Chen;Yuan Li;Ruru Wang

Reduction of bacterial adhesion on Ag-TiO2 coatings

减少 Ag-TiO2 涂层上的细菌粘附

• DOI: 10.1016/j.matlet.2018.02.044
• 发表时间: 2018
• 期刊: Materials Letters
• 影响因子: 3
• 作者: Liu Chen;Geng Lei;Yu Yifan;Zhang Yutong;Zhao Buyun;Zhang Shuai;Zhao Qi
• 通讯作者: Zhao Qi

Supplemental Material - Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters

补充材料-导尿管用抗菌聚多巴胺-羧甲基纤维素-银纳米颗粒水凝胶涂层的制备

• DOI: 10.25384/sage.22770595
• 发表时间: 2023
• 作者: Cai Y

Fungal-derived selenium nanoparticles and their potential applications in electroless silver coatings for preventing pin-tract infections.

• DOI: 10.1093/rb/rbac013
• 发表时间: 2022
• 期刊: REGENERATIVE BIOMATERIALS
• 影响因子: 6.7
• 作者: Liang, Xinjin;Zhang, Shuai;Gadd, Geoffrey Michael;McGrath, John;Rooney, David W.;Zhao, Qi
• 通讯作者: Zhao, Qi

Fabrication of antibacterial Fe3+-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters

• DOI: 10.1016/j.colsurfa.2023.131680
• 发表时间: 2023-05
• 期刊: Colloids and Surfaces A: Physicochemical and Engineering Aspects
• 作者: Jian-xiang Li;Han Yang;R. Gu;Yuhang Dong;Yong Cai;Qi Zhao;Yao-Cyong Chen;Mi Huang