Influence of Surface Properties of New Biomaterials for Catheters on Bacterial Adhesion in Urine

导管用新型生物材料表面特性对尿液中细菌粘附的影响

• 批准号: EP/P00301X/1
• 负责人: Qi Zhao
• 金额: $ 63.88万
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2016
• 资助国家: 英国
• 起止时间: 2016 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=EP%2FP00301X%2F1
• 关键词: Influence; Surface; Properties; New; Biomaterials

Catheter-associated urinary tract infection (CAUTIs) and encrustation have a severe impact on human health and health care costs. To date only a few types of antimicrobial coated catheters have come into clinical use, including a nitrofural-impregnated catheter, a silver alloy-hydrogel coated catheter, and a PTFE-coated catheter. Clinical trials show that these coated catheters give only an insignificant reduction in CAUTIs or encrustation formation. It is well known that both TiO2 and Ag nanoparticles exhibit bactericidal properties. However Ag nanoparticles with diameters less than 200 nm tend to aggregate spontaneously, and their stability in air, water or sunlight is not good enough for long-term application, which decreases their antibacterial performance. To solve these problems, a range of different Ag-TiO2 composite nanoparticles have been developed without aggregation. It has been demonstrated that both core-shell Ag@TiO2 nanoparticles (Ag nanoparticles coated with a thin shell of TiO2) and spherical Ag/TiO2 nanoparticles (TiO2 nanoparticles coated with Ag nanoparticles) exhibit synergistic bactericidal activities with long lasting action period in both light and dark conditions, compared with Ag and TiO2 nanoparticles. The Ag nanoparticles in Ag@TiO2 or Ag/TiO2 nanocomposites enable to active visible light excitation of TiO2, and can highly improve photocatalytic inactivation of bacteria. In this proposal two different types of Ag@TiO2-PTFE coated and Ag/TiO2-PTFE coated catheters will be developed by a so-gel technique. The two new types of coatings combine the excellent antibacterial properties of Ag@TiO2 and Ag/TiO2 nanoparticles and the non-stick properties of PTFE nanoparticles. The interactions between selected bacteria and the coated catheters in urine will be calculated and measured in order to find out optimum surface conditions of the coatings. The anti-bacterial and anti-encrustation performance of the coated full-length catheters in urine will be evaluated using bladder models that mimic in vivo environments of the human bladder for a catheter, and compared with existing silver-hydrogel coated catheter, PTFE coated catheter and uncoated silicone catheter. The cytotoxicity assays of the coated catheters will also be performed. After the project we will collaborate with local hospitals and a catheter manufacture to undertake clinical trials.

与导管相关的尿路感染（CAUTIS）和包裹对人类健康和医疗保健成本有严重影响。迄今为止，只有几种类型的抗菌涂层导管已经进入临床用途，包括硝化导管，含有硝化的导管，银合金 - 混合胶质涂层导管和PTFE涂层导管。临床试验表明，这些涂层的导管仅使小肠或包裹形成的降低微不足道。众所周知，TiO2和Ag纳米颗粒都表现出杀菌特性。但是，直径小于200 nm的Ag纳米颗粒倾向于自发聚集，并且它们在空气，水或阳光下的稳定性不足以长期施用，从而降低了其抗菌性能。为了解决这些问题，已经开发了一系列不同的AG-TIO2复合纳米颗粒，而没有聚集。已经证明，芯壳Ag@TiO2纳米颗粒（涂有薄的TiO2壳的Ag纳米颗粒）和球形AG/TiO2纳米颗粒（带有Ag纳米颗粒的TiO2纳米颗粒）在与AG和Tio的持久性作用相比，表现出具有延续的动作量，与AG和Tio相比，与持续的作用相比，与AG和TIO相比，均表现出了持久的作用。 Ag@TiO2或Ag/TiO2纳米复合材料中的Ag纳米颗粒可使TiO2的主动可见光激发，并且可以高度改善细菌的光催化失活。在此提案中，将通过So-Gel技术开发两种不同类型的AG@Tio2-Ptfe涂层和AG/Tio2-Ptfe涂层导管。两种新类型的涂层结合了AG@TiO2和Ag/Tio2纳米颗粒的出色抗菌特性以及PTFE纳米颗粒的不粘特性。将计算并测量选定细菌与尿液中涂层导管之间的相互作用，以找出涂层的最佳表面条件。将使用模仿人膀胱的体内环境中的膀胱模型来评估尿液中涂层全长导管的抗菌和抗肿瘤性能，并与现有的银 - 羟基凝胶导管，PTFE镀膜导管和未涂层的硅胶导管进行比较。还将进行涂层导管的细胞毒性测定。该项目结束后，我们将与当地医院和导管制造合作进行临床试验。

项目成果

Antibacterial AgNPs-PAAm-CS-PVP nanocomposite hydrogel coating for urinary catheters

• DOI: 10.1016/j.eurpolymj.2023.112260
• 发表时间: 2023-09
• 期刊: European Polymer Journal
• 影响因子: 6
• 作者: Yongwei Cai;Han Yang;Jianxiang Li;R. Gu;Yuhang Dong;Qi Zhao;Yao-Cyong Chen;Yuan Li;Ruru Wang

Reduction of bacterial adhesion on Ag-TiO2 coatings

减少 Ag-TiO2 涂层上的细菌粘附

• DOI: 10.1016/j.matlet.2018.02.044
• 发表时间: 2018
• 期刊: Materials Letters
• 影响因子: 3
• 作者: Liu Chen;Geng Lei;Yu Yifan;Zhang Yutong;Zhao Buyun;Zhang Shuai;Zhao Qi
• 通讯作者: Zhao Qi

Supplemental Material - Fabrication of antibacterial polydopamine-carboxymethyl cellulose-Ag nanoparticle hydrogel coating for urinary catheters

补充材料-导尿管用抗菌聚多巴胺-羧甲基纤维素-银纳米颗粒水凝胶涂层的制备

• DOI: 10.25384/sage.22770595
• 发表时间: 2023
• 作者: Cai Y

Fungal-derived selenium nanoparticles and their potential applications in electroless silver coatings for preventing pin-tract infections.

• DOI: 10.1093/rb/rbac013
• 发表时间: 2022
• 期刊: REGENERATIVE BIOMATERIALS
• 影响因子: 6.7
• 作者: Liang, Xinjin;Zhang, Shuai;Gadd, Geoffrey Michael;McGrath, John;Rooney, David W.;Zhao, Qi
• 通讯作者: Zhao, Qi

Fabrication of antibacterial Fe3+-carboxymethylcellulose-polyacrylamide-Ag nanoparticles hydrogel coating for urinary catheters

• DOI: 10.1016/j.colsurfa.2023.131680
• 发表时间: 2023-05
• 期刊: Colloids and Surfaces A: Physicochemical and Engineering Aspects
• 作者: Jian-xiang Li;Han Yang;R. Gu;Yuhang Dong;Yong Cai;Qi Zhao;Yao-Cyong Chen;Mi Huang