LIPOPROTEIN ANALYZER BASED ON PRINCIPLES OF FLOW FFF

基于 FFF 流动原理的脂蛋白分析仪

• 批准号: 3502465
• 负责人: MARCIA E HANSEN
• 金额: $ 5万
• 依托单位: FFFRACTIONATION, LLC
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-06-15 至 1994-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3502465
• 关键词: biomedical equipment development; blood fractionation; blood lipoprotein; clinical biomedical equipment; high density lipoproteins; low density lipoprotein; physical separation; very low density lipoprotein

DESCRIPTION: (Adapted from the applicant's abstract) Development of a Lipoprotein Analyzer system is proposed for rapid analysis of and characterization of the HDL, LDL, and VLDL profiles of human blood plasma. Preliminary results using a prototype system demonstrated the potential of the proposed system: the HDL, and VLDL fractions were clearly separated, plasma samples were analyzed without sample pre- treatment, and differences in lipoprotein profiles were noted for different patients.Analyses times were between 20 minutes and 1 hour. Determination of accurate particle size of the lipoprotein complexes also demonstrated the unique capabilities of this system. The separation strategy of the proposed Lipoprotein Analyzer is based on Flow field- flow fractionation, (Flow FFF) an elution based technique. This methodology is capable of high resolution measurements and is bio- compatible for analysis of biological macromolecules ranging from proteins up to chromosomes and cells. Thus this instrumentation is potentially a powerful and effective tool for rapid and direct measurement of the entire subfraction set of the lipoprotein profile. The applicants propose to further study, optimize, and develop Flow FFF so as to ultimately generate a rugged, routine technique. They expect this development will provide a more expedient and less expensive method for lipoprotein characterization than currently available.

描述：（改编自申请人的摘要）开发 提出了脂蛋白分析仪系统，用于快速分析和 HDL，LDL和VLDL的表征人类血液的特征 等离子体。 使用原型系统的初步结果证明了 拟议系统的潜力：HDL和VLDL级分是 明确分离，分析了血浆样品，而没有样品前样品 治疗和脂蛋白谱的差异被发现 不同的患者时间在20分钟至1小时之间。 确定脂蛋白复合物的精确粒径 还展示了该系统的独特功能。 分离 提出的脂蛋白分析仪的策略是基于流场 - 流分馏，（流FFF）基于洗脱技术。 这 方法论能够进行高分辨率测量，并且是生物的 兼容用于分析生物大分子的分析 蛋白质直至染色体和细胞。 因此，这种仪器是 可能是快速，直接的强大而有效的工具 测量脂蛋白剖面的整个亚剖分集。 申请人建议进一步研究，优化和发展流量FFF 以最终产生坚固的常规技术。 他们期望 这种发展将提供更便捷，更便宜的方法 对于脂蛋白表征，比当前可用的。