HERPESVIRIDAE INFECTIONS & THE PROGRESSION OF AIDS

疱疹病毒感染

• 批准号: 3488934
• 负责人: FLOYD E TAUB
• 金额: $ 5万
• 依托单位: DIGENE DIAGNOSTICS, INC.
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-05-01 至 1990-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3488934
• 关键词: AIDS /HIV diagnosis; AIDS /HIV test; Alphaherpesvirinae; Betaherpesvirinae; Epstein Barr virus; HIV infections; Herpesviridae disease; Pneumocystis pneumonia; diagnosis design /evaluation; diagnosis quality /standard; histology; human tissue; in situ hybridization; nucleic acid probes; oral leukoplakia; rapid diagnosis; viral pneumonia

AIDS is a clinical syndrome resulting from the interaction of a wide variety of pathogens with the immune system. The classic progression is from HIV exposure to seroconversion to ARC to AIDS. Accurate diagnosis of pathogens other than HIV is necessary to determine the progression of disease and appropriate therapy. Also, evidence suggests that viral interaction accelerates the progression from ARC to AIDS and death. If so, rapid diagnosis of these viruses os of extreme importance. DNA probes directly linked to enzymes can be quickly, easily, and effectively used to diagnose and quantify the presence of these viral pathogens, regardless of the status of the immune responses, antigen masking, or therapeutic intervention. In Phase I, DIGENE's directly labeled DNA probes will be used in rapid in situ hybridizations to detect the presence of viruses in AIDS-associated disorders, such as hairy leukoplakia, Kaposi's sarcoma, other skin disease, and pneumonia. The quantity and histologic location of these viruses will be investigated. Phase II would apply these findings by studying the pathogenesis of various AIDS-associated disorders and the significance of herpesviruses on HIV replication to determine if various antiviral therapies decrease viral replication.

艾滋病是由A的相互作用引起的临床综合征 免疫系统多种病原体。 经典 进展是从HIV暴露于血清转化到ARC到AIDS的进展。 需要对HIV以外的病原体进行准确诊断 确定疾病的进展和适当的治疗。 另外，有证据表明病毒相互作用加速了 从弧到艾滋病和死亡的发展。 如果是这样，请快速诊断 这些病毒OS非常重要。 直接链接到酶的DNA探针可以快速，容易，并且可以 有效地用于诊断和量化这些 病毒病原体，无论免疫反应的状态如何 抗原掩盖或治疗干预。 在第一阶段，Digene的直接标记的DNA探针将在 快速的原位杂交检测病毒的存在 艾滋病相关的疾病，例如毛茸茸的白斑，卡波西的疾病 肉瘤，其他皮肤病和肺炎。 数量和 这些病毒的组织学位置将进行研究。 第二阶段将通过研究发病机理应用这些发现 各种艾滋病相关的疾病以及 艾滋病毒复制上的疱疹病毒，以确定各种抗病毒 疗法减少病毒复制。