INTESTINAL ABSORPTION OF MINERAL IONS

肠道对矿物质离子的吸收

• 批准号: 3482878
• 负责人: ROBERT WASSERMAN
• 金额: $ 27.11万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3482878
• 关键词: adenosine triphosphate; brush border membrane; calcium transporting ATPase; calmodulin; chick embryo; chickens; cholecalciferol; dietary calcium; dietary trace element; gastrointestinal epithelium; gastrointestinal nutrient absorption; immunochemistry; immunofluorescence technique; ion transport; magnesium; membrane permeability; membrane proteins; nutrition related tag; protein sequence; radioimmunoassay; vitamin D deficiency

The proposed research represents a continuation of the investigation on the transport of calcium and other mineral ions across the intestine. Emphasis will be given to vitamin D and other factors that control mineral ion absorption and intestinal function. Specific studies to be undertaken within this grant period are, as follows: a) Continued investigation of the function vitamin D and calbindin-D28K in Ca2+ transport across epithelial membranes and continued investigation of the ATP-dependent calcium pump, with emphasis on the responsiveness of the pump to vitamin D and calbindin-D28K; b) Investigate the state and localization of calcium and other mineral ions during the course of transepithelial transport using spectrofluorometry with fluorescent calcium indicator dyes (fura 2; quin 2) for free Ca2+, and ion microscopy for total Ca2+. Chlortetracycle, another fluorescent Ca2+ indicator, will be examined as a probe for intravesicular Ca2+; c) Investigate the mechanism or mechanisms of magnesium transport across the intestine and other epithelial tissues, and the role of vitamin D and other affectors on its transport and metabolism; d) Investigate the effect of vitamin D on the function of the intestinalis nerve of the chick, the latter shown to contain vitamin D-responsive calbindin-D28K, using various neurophysiological and biochemical approaches. e) Continued studies on the effect of E. coli heat stable enterotoxin and cGMP on the Ca2+ permeability of the enterocyte, and f) Continued studies on the mechanism of selenite absorption and the relation of vitamin D-dependent selenite absorption and the relation of vitamin D-dependent selenite transport to other anion transport systems.

拟议的研究代表了对有关的调查的延续 钙和其他矿物离子在整个肠道上的运输。 强调 将给予维生素D和其他控制矿物离子的因素 吸收和肠功能。 要进行的具体研究 在此赠款期内，如下：a）继续调查 CA2+跨过CA2+运输的功能维生素D和Calbindin-D28K 上皮膜并继续研究ATP 钙泵，重点是泵对维生素的反应性 D和Calbindin-D28K; b）研究钙的状态和定位 和其他矿物离子在跨层次运输过程中使用 具有荧光钙指示剂染料的光谱荧光测定法（Fura 2; Quin 2） 用于总Ca2+的自由Ca2+和离子显微镜。 ChlorteTracycle，另一个 荧光Ca2+指示器将被检查为探针的探针 Ca2+; c）研究镁转运的机制或机制 跨肠道和其他上皮组织，维生素的作用 D和其他情感者对其运输和代谢； d）调查 维生素D对雏鸡肠道神经功能的作用， 后者使用的是含有维生素D-响应性calbindin-d28k的，使用 各种神经生理和生化方法。 e）继续 大肠热稳定肠毒素和CGMP对大肠杆菌热的研究对 CA2+肠球细胞的渗透性，f）继续研究 亚硒酸盐吸收的机理和维生素D依赖性的关系 亚硒酸盐的吸收和维生素D依赖性亚硒酸盐的关系 运输到其他阴离子运输系统。