INTESTINAL ABSORPTION OF MINERAL IONS

肠道对矿物质离子的吸收

• 批准号: 3224449
• 负责人: ROBERT WASSERMAN
• 金额: $ 29.77万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3224449
• 关键词: X ray crystallography; adenosinetriphosphatase; alkaline phosphatase; basolateral membrane; brush border membrane; calcium transporting ATPase; calmodulin; chick embryo; chickens; cholecalciferol; chromatography; dietary calcium; dietary trace element; gastrointestinal nutrient absorption; gel filtration chromatography; immunochemistry; immunofluorescence technique; liposomes; membrane proteins; nuclear magnetic resonance spectroscopy; nutrition related tag; phospholipids; phosphorus; protein sequence; radioimmunoassay; vitamin D deficiency; vitamin metabolism

The objectives of the proposed research constitutes a continuation of investigations on the transport of calcium and other mineral ions across the epithelium of the intestine. Emphasis will be given to different aspects of the problems of vitamin D action. Specific aims are to: (a) determine the amino acid sequence of chick CaBP and its interactions, (b) investigate in detail alterations in phopholipid metabolism as related to 1,25(OH)2D3 action, (c) to study possible 1,25(OH)2D3-mediated changes in membrane fluidity and mobility of membrane components, (d) initiate studies on the use of epithelial cell lines to investigate aspects of 1,25(OH)2D3 action, (e) investigate the properties and characteristics of the ATP-dependent basal-lateral pump, (f) identify and investigate the properties and function of specific 1,25(OH)2D3-modulated proteins and lipids, (g) examine hormonal effects in a model in which chicks are given 1,25(OH)2D3 as their only source of vitamin D3, and (h) to determine possible alterations in calmodulin behavior in calcium, phosphorus and vitamin D3-deficiency.

拟议研究的目标构成了 对钙和其他矿物离子跨越的调查 肠上皮。 重点将给予不同的 维生素D作用问题的各个方面。 具体目的是：（a） 确定雏鸡Cabp的氨基酸序列及其相互作用，（b） 详细研究与苯酚代谢有关的改变与 1,25（OH）2D3动作，（c）研究可能的1,25（OH）2d3介导的变化 膜成分的膜流动性和迁移率，（d）启动研究 关于使用上皮细胞系研究1,25（OH）2d3的方面 作用，（e）研究该特性和特征 依赖ATP的基底泵，（f）识别和研究 特定1,25（OH）2d3调节蛋白的特性和功能和功能 脂质，（g）在给出小鸡的模型中检查激素效应 1,25（OH）2d3是其唯一的维生素D3来源，并且（H）确定 钙，磷和 维生素D3缺乏。