NON-PROTEIN AMINO ACID BIOCHEMISTRY AND TOXICITY

非蛋白质氨基酸生物化学和毒性

• 批准号: 3225714
• 负责人: GERALD A ROSENTHAL
• 金额: $ 7.92万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-07-01 至 1988-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3225714
• 关键词: Leguminoseae; aminoacid analog; aminoacid biosynthesis; antimetabolites; arginine; colorimetry; cytotoxicity; electron microscopy; electron spin resonance spectroscopy; erythrocyte membrane; gel electrophoresis; membrane structure; nucleic acid metabolism; ornithine; plants; radiotracer; tissue /cell culture; urea cycle

Higher plants synthesize a diversified array of non-protein amino acids many of which bear marked structural analogy to the amino acids of mammalian metabolism. As a result, these secondary plant constituents are usually highly toxic and exhibit pronounced antimetabolic properties in animals. A particular group of naturally occurring non-protein amino acids, namely L-canavanine, L-canaline, and O-ureido-L-homoserine, are structurally related to the intermedates of the Krebs-Henseleit ornithine-urea cycle of animals. This group of non-protein amino acids will be utilzed as a model for understanding the biochemical basis for the toxicity and mode of action of these toxic amino acids. Emphasis will be placed on improving the utility of these compounds in fundamental biochemical studies.

高等植物合成多种非蛋白质氨基酸 它们与哺乳动物新陈代谢的氨基酸具有明显的结构相似性。 因此，这些次生植物成分通常具有剧毒且 在动物中表现出明显的抗代谢特性。 一组特定的天然存在的非蛋白质氨基酸，即 L-刀豆氨酸、L-刀豆氨酸和 O-脲基-L-高丝氨酸在结构上与 动物克雷布斯-亨塞莱特鸟氨酸-尿素循环的中间产物。 这 一组非蛋白质氨基酸将被用作理解的模型 这些有毒氨基的毒性和作用方式的生化基础 酸。 重点将放在提高这些化合物在 基础生化研究。

项目成果

Purification and characterization of the higher plant enzyme L-canaline reductase.

高等植物酶 L-canaline 还原酶的纯化和表征。

• 发表时间: 1992-03-01
• 影响因子: 11.1
• 作者: Rosenthal; G A
• 通讯作者: G A

Metabolism of L-[guanidinooxy-14C]canavanine in the rat.

L-[胍基氧基-14C]刀豆氨酸在大鼠体内的代谢。

• 发表时间: 1987-12
• 影响因子: 3.8
• 作者: Thomas, D A;Rosenthal, G A
• 通讯作者: Rosenthal, G A

Ductal cancers of the pancreas frequently express markers of gastrointestinal epithelial cells.

胰腺导管癌经常表达胃肠道上皮细胞的标记物。

• 发表时间: 1990-06
• 影响因子: 29.4
• 作者: Sessa, F;Bonato, M;Frigerio, B;Capella, C;Solcia, E;Prat, M;Bara, J;Samloff, I M
• 通讯作者: Samloff, I M

Aberrant, canavanyl protein formation and the ability to tolerate or utilize L-canavanine.

异常的刀豆蛋白形成以及耐受或利用 L-刀豆氨酸的能力。

• 发表时间: 1987-05-15
• 作者: Rosenthal, G A;Berge, M A;Bleiler, J A;Rudd, T P
• 通讯作者: Rudd, T P