DEVELOPMENTAL MORPHOGENETICS OF THE MOUSE DENTITION

小鼠牙列的发育形态学

• 批准号: 3425693
• 负责人: KENNETH M. WEISS
• 金额: $ 4.9万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3425693
• 关键词: ameloblasts; cell cell interaction; cell differentiation; collagen; complementary DNA; computer graphics /printing; dental development; dental structure; developmental genetics; embryo /fetus tissue /cell culture; enamel organ; extracellular matrix; gene expression; genetic library; histochemistry /cytochemistry; homeobox genes; in situ hybridization; laboratory mouse; longitudinal animal study; mammalian embryology; messenger RNA; molecular cloning; neural crest; northern blottings; odontoblasts; polymerase chain reaction; regulatory gene; structural genes; tissue /cell preparation; tooth

The mammalian dentition is a complex organ whose normal development is of major health importance, yet whose dysgenesis and deterioration have been grossly under-studied. The dentition is also an important model system for the genetics of complex, segmented organs that develop through tissue interactions. This research-initiation project will investigate regulatory gene expression patterns in mouse tooth germs. First, mRNA obtained from 10. 5 daytooth germs will be screened by polymerase chain reaction (PCR) methods to amplify all expressed homeotic (Hox) genes. The amplified products will be sequenced and identified; previously unidentified genes will be cloned and mapped using a mouse genome library. Similar procedures will be used for Int-2, Collagen I, and tenascin (and closely homologous genes), that are involved in the control of cell differentiation and extra-cellular matrix production. In situ hybridization will be done on sections of tooth germs at various developmental ages and in morphologically distinct parts of each tooth, to develop a spatio-temporal regulatory expression map for genes identified as above. Finally, work will be done to develop PCR-based methods for improved sensitivity in microhistological expression analysis. This study will lay the ground work for systematic investigations of cell-cell interaction, neural crest function, and the morphogenetics of mammalian teeth, with direct application to the human dentition and to dental dysgenesis. This is responsive to NIDR's Small Grants Program, as a research retraining initiative for Kenneth M. Weiss, and an extension of ongoing methods to a new area for Frank H. Ruddle.

哺乳动物牙列是一个复杂的器官，其正常发育是 主要健康的重要性，但其失调和恶化已经 严重研究。 牙列也是遗传学的重要模型系统 通过组织相互作用而发展的复杂的分段器官。 该研究生动项目将调查监管基因 小鼠牙齿中的表达模式。 首先，从10。5获得的mRNA 日齿细菌将通过聚合酶链反应（PCR）方法筛选 放大所有表达的同源（HOX）基因。 放大产品将 被测序并确定；以前未识别的基因将被克隆 并使用鼠标基因组库映射。 将使用类似的程序 对于Int-2，胶原蛋白I和Tenascin（以及非常同源的基因）， 参与细胞分化和细胞外的控制 矩阵生产。 原位杂交将在各种牙齿细菌的各个部分进行 发育年龄和每个牙齿的形态不同部分， 为鉴定为鉴定的基因开发时空调节表达图 多于。 最后，将进行开发基于PCR的方法的工作 在微观组织学分析中提高了灵敏度。 这项研究将为系统研究的基础工作奠定 细胞 - 细胞相互作用，神经波峰功能和形态学学 哺乳动物牙齿，直接应用于人类牙列和 牙科发病障碍。 这是对NIDR的小赠款计划的响应 Kenneth M. Weiss的倡议，以及正在进行的方法扩展到 Frank H. Ruddle的新区域。