MODELING DNA DIVERSITY IN CARDIOVASCULAR HEALTH/DISEASE

心血管健康/疾病中的 DNA 多样性建模

• 批准号: 2735375
• 负责人: KENNETH M. WEISS
• 金额: $ 22.78万
• 依托单位: PENNSYLVANIA STATE UNIVERSITY, THE
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-15 至 2002-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2735375
• 关键词: DNA; blood pressure; cardiovascular disorder; cardiovascular disorder epidemiology; cholesterol; clinical research; cooperative study; genetic models; genetic polymorphism; genotype; high density lipoproteins; human genetic material tag; human population genetics; human subject; model design /development; nucleic acid sequence; racial /ethnic difference; triglycerides

DESCRIPTION (adapted from the applicants abstract) Project 1 is a research component of a Cooperative Research Program involving two other research components, Project 2, Weiss, PI and Project 3, Sing, PI. One of the most complex and challenging problems in human biology and medicine is defining the relationship between DNA sequence variation and interindividual variation in quantitative risk factors for complex diseases having a multifactorial etiology. As their knowledge about the basic human DNA sequence increases, so will their need to define the range of natural variation n human populations and to explore the relationship between nucleotide diversity and phenotype variation in measures of human health. The goal of Project 1 is to identify and measure DNA sequence variation in 13 genes that play a central role in key physiological functions involved in the development of cardiovascular disease (CVD), i.e. genes involved in lipid metabolism. Project 1 will apply state-of-the-art automated fluorescence-based sequencing and high-throughput DNA genotyping methods to uncover and assess DNA sequence variation in three human populations: non-Hispanic Whites from Rochester, MN (low CVD risk), African-Americans from Jackson, MS (intermediate CVD risk) and non-Hispanic Whites from North Karelia, Finland (high CVD risk). These studies will provide an unprecedented

描述（改编自申请人摘要）项目1是一项研究 合作研究计划的组成部分，涉及其他两项研究 组件，项目2，Weiss，Pi和Project 3，Sing，Pi。 人类生物学中最复杂，最具挑战性的问题之一 医学正在定义DNA序列变化与 复杂疾病的定量风险因素的个体变化 具有多因素病因。 作为他们对基本人类的知识 DNA序列增加，因此他们需要定义自然范围 变异n人口并探索 人类健康量度的核苷酸多样性和表型变化。 项目1的目的是识别和测量DNA序列变化 13个在涉及的关键生理功能中起着核心作用的基因 心血管疾病（CVD）的发展，即涉及的基因 脂质代谢。 项目1将应用最新的自动化 基于荧光的测序和高通量DNA基因分型方法 发现并评估三个人群的DNA序列变化： 来自明尼苏达州罗切斯特的非西班牙裔白人（低CVD风险），非裔美国人 来自MS杰克逊（中级CVD风险）和北部的非西班牙白人 芬兰Karelia（高CVD风险）。 这些研究将提供 空前的