ANTIBODY PROBES OF A PRESYNAPTIC CALCIUM CHANNEL

突触前钙通道的抗体探针

• 批准号: 3418452
• 负责人: CAMERON B GUNDERSEN
• 金额: $ 16.43万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1996-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3418452
• 关键词: Torpedo; Xenopus oocyte; alternatives to animals in research; calcium channel; electron microscopy; hybridomas; immunoaffinity chromatography; immunoprecipitation; laboratory mouse; laboratory rabbit; molecular cloning; monoclonal antibody; neural transmission; peptides; protein purification; protein structure; protein structure function; synapses; western blottings

The long-term goal of this project is to increase our understanding of synaptic communication in the nervous system. The molecular mechanism by which neurotransmitters are released at chemical synapses is still not resolved. However, it is known that calcium channels of nerve endings are vital for regulating this process. This work directly addresses questions concerning the structure and properties of presynaptic calcium channels, and it will lead to the production of reagents that should be of long-term utility for studying these proteins. Specifically, this project entails the production of monoclonal and polyclonal antibodies against a candidate subunit of a presynaptic calcium channel that was recently cloned by this laboratory. These antibodies will be used to purify this protein and other candidate calcium channel subunits. These antibodies will also be used for the immunolocalization of this antigen in tissues and subcellular fractions. These investigations should dramatically improve our knowledge of presynaptic calcium channels and how they contribute to normal synaptic function. This is a crucial prerequisite for applied studies of how these channels are modulated by environmental and genetic factors in health and disease.

该项目的长期目标是增加我们对 神经系统中的突触通信。 分子机制 哪些神经递质在化学突触中释放 解决。 但是，众所周知，神经结尾的钙通道 对于调节这一过程至关重要。 这项工作直接解决 有关突触前钙的结构和特性的问题 渠道，这将导致应有的试剂生产 研究这些蛋白质的长期实用性。 具体来说，这是 项目需要产生单克隆和多克隆抗体 针对突触前钙通道的候选亚基 最近由该实验室克隆。 这些抗体将用于 净化该蛋白质和其他候选钙通道亚基。 这些 抗体也将用于该抗原的免疫定位 在组织和亚细胞级分中。 这些调查应该 极大地提高了我们对突触前钙通道的了解和 它们如何促进正常的突触功能。 这是至关重要的 对这些渠道如何调节这些渠道的应用的先决条件 健康和疾病中的环境和遗传因素。