RECONSTITUTION OF A NEURONAL TRANSMITTER RELEASE SYSTEM

神经递质释放系统的重构

• 批准号: 3074700
• 负责人: CAMERON B GUNDERSEN
• 金额: $ 5.41万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-06-01 至 1989-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3074700
• 关键词: acetylcholine; egg /ovum; fresh water environment; messenger RNA; motor neurons; neural information processing; neural plasticity; neurotransmitters; saltwater environment; synaptic vesicles

The goal of this project is to improve our understanding of the transmitter release process and the means of regulating presynaptic events in nerve cells. The approach entails the reconstitution of a neuronal transmitter release system using the oocytes of Xenopus laevis. These oocytes can be microinjected with a wide range of materials, and importantly, have been shown to translate exogenously supplied messenger RNA. The reconstruction of the release process involves three phases. First, oocytes will be injected with messenger RNA from electromotor neurons of Torpedo or Narcine. This step may be crucial for endowing oocytes with components needed to support the exocytotic discharge of transmitter. The oocyte will then be injected with synaptic vesicles isolated from the electroplax tissue of Torpedo or Narcine. Finally, the oocytes will be stimulated electrically or chemically to determine whether evoked release of transmitter (acetylcholine) can be detected. Success of this reconstitution effort will facilitate a detailed examination of the molecular components involved in transmitter secretion. The resulting observations may open new avenues to the study of biochemical correlates of synaptic plasticity. Moreover, a wide range of neurological deficits (eg., dementia, Parkinsonism, mental retardation) might be amenable to scrutiny in a new light.

该项目的目的是提高我们对发射器的理解 释放过程和调节神经突触前事件的手段 细胞。 该方法需要重组神经元发射器 使用爪蟾laevis的卵母细胞释放系统。 这些卵母细胞可以是 用广泛的材料进行了显微注射，重要的是 显示用于翻译外源提供的使者RNA。 重建 释放过程涉及三个阶段。 首先，卵母细胞将是 从鱼雷的电动神经元或 narcine。 此步骤对于将卵母细胞赋予成分至关重要 需要支持发射机的胞吐排放。 卵母细胞会 然后注入从电流分离的突触囊泡 鱼雷或纳尔辛的组织。 最后，将刺激卵母细胞 电或化学以确定是否诱发 可以检测到发射机（乙酰胆碱）。 成功的成功 重组努力将促进对 分子成分参与发射机分泌。 结果 观察可能为研究的生化相关性研究开辟新的途径 突触可塑性。 此外，多种神经缺陷（例如， 痴呆症，帕金森氏症，智力低下）可能需要审查 用新的角度。