OPTICAL RECORDING OF SYNAPTIC POTENTIAL INTEGRATION

突触电位整合的光学记录

• 批准号: 3414957
• 负责人: LAWRENCE B COHEN
• 金额: $ 6.1万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-09-01 至 1993-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3414957
• 关键词: Mollusca; axon; cell cell interaction; dendrites; electrical potential; electrophysiology; fluorescent dye /probe; ganglions; neural information processing; neurons; neuropsychology; saltwater environment; single cell analysis; soma; synapses

The long term goal of the proposal is to explain the neuronal bases of simple forms of behavior at the level of signal processing in all of the elements of the functional neuronal network. Toward this goal, we would first try to achieve simultaneous recording of the action potentials and subthreshold signals in both cell bodies and processes in a restricted number of neurons using preparations in which simultaneous recording of action potentials from most neuronal cell bodies (but not processes)is already possible. This study would be based on experiments using multi-site optical recording techniques. Optical recording of subthreshold potential changes from small neuronal processes is still exceedingly difficult because of the small signal size relative to noise. At present it can only be used under most favorable and thus very limited experimental conditions. The improvement in sensitivity that we hope to obtain will be based on two approaches. First we would exploit the idea of limiting the application of impermeant voltage-sensitive dyes to a restricted number of neurons in the network and not stain the whole structure. This may be accomplished by intracellular application of dyes into cell bodies. Intracellular staining would improve spatial resolution of optical recording as well as signal-to-noise ratio. Second, we propose to continue the search for new dyes that would give better optical signals after either extra- or intracellular application. We propose to test analogs of most successful dyes from different categories of voltage sensitive compounds and to use several different invertebrate preparations that are especially suitable for optical recording studies of neuronal correlates of behavior. With the best dye/preparation combination we then propose to develop the recording and analysis of signal integration at the level of neuronal processes of individual cells within a functional network. The knowledge we obtain about how normal behavior is generated in these primitive animals will help us understand mental disorders such as depression, drug addiction or schizophrenia.

该提案的长期目标是解释 所有在所有信号处理层面的简单形式的行为形式 功能性神经元网络的元素。达到这个目标，我们会 首先尝试同时记录动作电位，并 细胞体和过程中的子阈值信号 使用制剂的神经元数量，其中同时记录 大多数神经元细胞体（但不是过程）的动作电位是 已经可能了。 这项研究将基于使用多站点光学记录的实验 技术。子阈值的光学记录小小的变化 神经元过程仍然非常困难，因为很小 信号大小相对于噪声。目前，它只能在大多数下使用 有利，因此实验条件非常有限。改进 我们希望获得的敏感性将基于两种方法。第一的 我们将利用限制应用不理的想法 电压敏感的染料对网络中的神经元数量有限，并且 不染色整个结构。这可以通过细胞内完成 染料在细胞体中的应用。细胞内染色会改善 光学记录以及信噪比的空间分辨率。 其次，我们建议继续寻找将提供的新染料 细胞外或细胞内应用后更好的光学信号。我们 建议测试来自不同类别的大多数成功染料的类似物 电压敏感化合物，并使用几种不同的无脊椎动物 特别适合光学记录研究的制剂 行为的神经元相关。 使用最好的染料/制剂组合，我们建议开发 在神经元水平上的信号积分记录和分析 功能网络中各个单元的过程。我们的知识 获取这些原始动物中如何产生正常行为的信息 将帮助我们理解诸如抑郁症，吸毒等精神障碍 或精神分裂症。