DEVELOPING MUSCLE SPINDLE

发展肌梭

• 批准号: 3411284
• 负责人: JAN KUCERA
• 金额: $ 8.17万
• 依托单位: BOSTON UNIVERSITY MEDICAL CAMPUS
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-02-01 至 1991-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3411284
• 关键词: afferent nerve; cell differentiation; electron microscopy; evolution; histochemistry /cytochemistry; innervation; laboratory rat; microscopy; motor neurons; myofibrils; myogenesis; neuroanatomy; stretch receptors; tetrodotoxin

This application proposes a series of experimental neuroanatomical studies on the development of muscle spindles in the rat soleus muscle. It has three main objectives: 1) to examine the developmental mechanisms whereby spindles acquire the adult pattern of their sensory and motor neural connections; 2) to determine the role of sensory and motor neurons in the structural and histochemical differentiation of intrafusal fibers; and 3) to explore the mechanisms of the ontogenic effect of sensory neurons on spindle formation. Neural and muscular features of spindles in the soleus muscle will be compared by light and electron microscopy between developing and adult rats. In a complementary study, these features of developing spindles will also be compared between normal controls and animals subjected to experimental deafferentation and exposure to tetrodotoxin. These comparisons will be used to a) determine the temporal sequence of the development of sensory and motor innervation to spindles; b) establish whether transient, immature patterns of innervation procede the emergence of the adult pattern of neural connections of spindles; c) determine whether the motor innervation can direct the differentiation of developing spindles in the absence of sensory innervation; and d) explore whether nerve activity could mediate the ontogenetic effects of sensory axons on spindle formation. The proposed study aims at providing new insights into the process of formation and differentiation of mammalian muscle spindles. It will also explore nerve-muscle interactions during the development of a highly specialized class of muscle fibers - the intrafusal fibers of muscle spindles.

该应用提出了一系列实验 关于肌肉纺锤体发展的神经解剖学研究 大鼠比目鱼肌。 它有三个主要目标：1） 检查旋转获取的发育机制 他们的感觉和运动神经连接的成年模式； 2）确定感觉和运动神经元在 恒星纤维的结构和组织化学分化； 3）探讨 纺锤体形成的感觉神经元。 比目鱼肌中主轴的神经和肌肉特征将 通过发育之间的光和电子显微镜进行比较 和成年大鼠。 在一项互补的研究中，这些特征 也将在正常之间进行比较 受到实验性脱落的对照和动物 并暴露于四毒素。 这些比较将用于 a）确定开发的时间顺序 感官和运动神经支配； b）确定是否 瞬态，未成熟的神经支配过程 纺锤体神经联系的成年模式的出现； c）确定电动机神经是否可以指导 在没有感觉的情况下，开发纺锤体的差异 神经d）探索神经活动是否可以介导 感觉轴突对纺锤体形成的个体发育作用。 拟议的研究旨在为过程提供新的见解 哺乳动物肌肉纺锤体的形成和分化。 它还将在 开发高度专业的肌肉纤维 - 肌肉纺锤体的鼻腔内纤维。