DEVELOPMENT OF SYNAPSE

突触的发育

• 批准号: 3407263
• 负责人: Hsiao-Ming B Peng
• 金额: $ 14.12万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3407263
• 关键词: Anura; Xenopus; acetylcholine; cell differentiation; electron microscopy; fresh water environment; immunocytochemistry; innervation; laboratory mouse; larva; myogenesis; neural information processing; neural transmission; neurogenesis; neuromuscular junction; nicotinic receptors; synapses; synaptic vesicles; tissue /cell culture

At the neuromuscular junction, the transmission of signal from the nerve to the muscle which causes muscle contraction is mediated by the release of acetylcholine from the nerve terminal and the sensing of this molecule by the receptors in the muscle. To gain insight into the formation of the neuro-muscular junction, this study will use physiological and structural techniques to observe the development of the acetylcholine receptors in the muscle cell and the development of the acetylcholine release mechanism in the nerve terminal in tissue culture and the interaction between the nerve and the muscle during innervation. We are particularly interested in the information that the nerve cell conveys to the muscle in effecting the accumulation of acetylcholine receptors in the postjunctional membrane and the mechanism that the muscle cell uses in generating such a receptor accumulation. On the presynaptic side, we are interested in the differentiation of the nerve terminal induced by its target and the mechanism of the formation of presynaptic vesicle clusters. Besides conventional nerve-muscle cocultures, we will also study the formation of the acetylcholine receptor clusters and synaptic vesicle clusters induced by basic polypeptide-coated latex beads. Previous studies from this laboratory have shown that these compounds can induce a local pre- and postsynaptic-type differentiation. These studies are aimed at providing a fundamental understanding of the development of the muscle during innervation. This knowledge is essential for the elucidation of the cause of many neuromuscular disorders.

在神经肌肉连接处，信号从 神经介导导致肌肉收缩的肌肉是由 从神经末端释放乙酰胆碱和 通过肌肉中的受体传感该分子。 获得 深入了解神经肌肉结的形成，这是 研究将使用生理和结构技术观察 肌肉细胞中乙酰胆碱受体的发展 以及乙酰胆碱释放机制的发展 组织培养中的神经末端以及 神经过程中神经和肌肉。 我们特别是 对神经细胞传达给的信息感兴趣 肌肉影响乙酰胆碱受体在 后膜和肌肉细胞的机制 用于产生这种受体积累的用途。 在 突触前的一面，我们对差异化感兴趣 神经末端是由其靶标诱导的 突触前囊泡簇的形成。 除了传统 神经肌肉共培养，我们还将研究 乙酰胆碱受体簇和突触囊泡簇 由碱性多肽涂层乳胶珠诱导。 先前的研究 从这个实验室表明，这些化合物可以诱导 局部和突触后型分化。 这些研究 旨在提供对 神经支配期间肌肉的发展。 这些知识是 阐明许多神经肌肉的原因必不可少的 疾病。