DEVELOPMENT OF SYNAPSE

突触的发育

• 批准号: 3407260
• 负责人: Hsiao-Ming B Peng
• 金额: $ 12.22万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1989-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3407260
• 关键词: acetylcholine; cell differentiation; electron microscopy; fresh water environment; histochemistry /cytochemistry; immunochemistry; innervation; larva; myogenesis; neural information processing; neural transmission; neuromuscular junction; synapses; synaptic vesicles; tissue /cell culture

At the neuromuscular junction, the transmission of signal from the nerve to the muscle which causes muscle contraction is mediated by the release of acetylcholine from the nerve terminal and the sensing of this molecule by the receptors in the muscle. To gain insight into the formation of the neuromuscular junction, this study will use physiological and structural techniques to observe the development of the acetylcholine receptors in the muscle cell and the development of the acetylcholine release mechanism in the nerve terminal in tissue culture and the interactions between the nerve and the muscle during innervation. We are particularly interested in the information that the nerve cell conveys to the muscle in effecting an accumulation of acetylcholine receptors in the postjunctional membrane and the mechanism that the muscle cell uses in generating such a receptor accumulation. Conversely, we are also interested in the differentiation of the nerve terminal induced by its target and the mechanism of the formation of presynaptic synaptic vesicle clusters. Besides conventional nerve-muscle cocultures, we will also study the formation of the acetylcholine receptor clusters and synaptic vesicle clusters induced by basic polypeptide-coated latex beads. Previous studies in this laboratory have shown that these compounds can induce a local pre- and postjunctional-type differentiation. These studies are aimed at providing a fundamental understanding of the development of the muscle during innervation. This knowledge is essential for the elucidation of the cause of many neuromuscular disorders.

在神经肌肉连接处，信号从神经传播到 引起肌肉收缩的肌肉通过释放介导 来自神经末端的乙酰胆碱以及该分子通过 肌肉中的受体。 洞悉形成 神经肌肉结，本研究将使用生理和结构 观察乙酰胆碱受体发展的技术 肌肉细胞和乙酰胆碱释放机制的发展 组织培养中的神经末端和神经之间的相互作用 和神经支配期间的肌肉。 我们对 神经细胞传达给肌肉的信息 乙酰胆碱受体在后膜中的积累和 肌肉细胞在产生这种受体中使用的机制 积累。 相反，我们也对差异化感兴趣 由其靶标诱导的神经末端及其形成机理 突触前突触囊泡簇。 除了传统 神经肌肉共培养，我们还将研究 乙酰胆碱受体簇和突触囊泡簇 基本的多肽涂层乳胶珠。 该实验室的先前研究 已经表明，这些化合物可以诱导局部和 后型分化。 这些研究旨在提供 对肌肉发育的基本理解 神经。 这种知识对于阐明原因至关重要 许多神经肌肉疾病。