MORPHOLOGIC STUDY OF HYPERTENSIVE CEREBROVASCULARITY

高血压脑血管形态学研究

• 批准号: 3401069
• 负责人: DIXON M MOODY
• 金额: $ 13.01万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3401069
• 关键词: basal ganglia; cerebral aneurysm; cerebrovascular disorders; cerebrovascular occlusions; histochemistry /cytochemistry; human tissue; hypertension; image processing; infarct; medulla oblongata; mesencephalon; microcirculation; spinal cord

We propose to use the higher resolving power and high contrast of an advanced state-of-the art small focal spot "soft" (low kvp) x-ray unit to study microvascular changes in brains from hypertensive primates and humans and to compare the changes seen with normative controls. Patients with hypertension have a greatly increased incidence of intracerebral hemorrhage and cerebral infarcts. The light microscope has been used extensively to study the microvascular changes in this disease process, but analysis is limited by tissue thickness, i.e., only a small piece if the vascular bed can seen in any one section. Examination of a long, conplex anatomical unit such as an artery-capillary-venule complex is extremely tedious using thin section light microcscope in the evaluation of a longitudinal structure which is to be followed from one plane to the other. Because of the penetrating power and depth of focus, X ray is uniquely capable of examining the vascular bed. Human Material: Specimens of brain tissue from patients with hypertension will be studied for microcirculatory changes from hypertension and compared with normals. Small areas form five specific locations of already fixed brains in addition to spinal cord will be prepared by the histochemical technique originated by the coprincipal investigator and examined by x-ray microscopy. Then these same blocks of tissue will be cut and subjected to a detailed neutopthological examination. Animal Material: Specimens of fixed brain tissue form hypertensive primates will also be prepared, stained by the alkaline phophatase method devised by the coprincipal investigator and examined using both light and x-ray microscopy for microvascular changes and then compared with normals. We will move beyond previous microvascular studies in two areas: a) use of an advanced newly developed, nondestructive testing type x-ray unit that will increase resolving power; b) we will not use capricious barium perfusion angiographic techniques, but will stian the vascular tree directly using a recently developed histochemical staining technique devised by the co-principal investigator. It is believed that this study will identify the premorbid anatomical alteration leading to intracerbral hemorrhage or infarct in patients with hypetension.

我们建议利用更高的分辨能力和高度对比度 先进的先进最先进的小焦点点“软”（低KVP）X射线单元 研究高血压灵长类动物和人类大脑的微血管变化 并比较所见的变化与规范控制。 患者 高血压大大增加了脑出血的发生率 和脑梗塞。 光学显微镜已广泛用于研究微血管 这种疾病过程的变化，但分析受组织的限制 厚度，即，如果在任何一张血管床中都可以看到一小块 部分。 检查一个长的，综合的解剖单元，例如 动脉毛细血管膜复合物使用薄截面极为乏味 在评估纵向结构中的光微观 从一个平面到另一个平面。 由于具有穿透力 X射线的焦点和焦点深度具有独特的能力检查血管 床。 人类材料：高血压患者的脑组织标本 将研究从高血压中进行微循环的变化，并比较 与正常人。 小区域形成五个已经固定的特定位置 除脊髓外，大脑还将通过组织化学制备 技术由副研究人员发起，并由X射线检查 显微镜。 然后，将切割这些相同的组织块并进行 详细的中心研究。 动物材料：固定脑组织的标本形成高血压 灵长类动物也将由碱性磷酸酶法染色准备 由联合研究人员设计，并使用光和 X射线显微镜用于微血管变化，然后与正常质量进行比较。 我们将超越两个领域的微血管研究：a）使用 一个高级新开发的无损测试X射线单元 将增加分辨力； b）我们不会使用反复无常的钡 灌注血管造影技术，但会Stian Stian血管树 直接使用最近开发的组织化学染色技术 由联合首席研究员设计。 据信这项研究将识别出前解剖学 改变导致脑内出血或梗死的患者 高血压。