MORPHOLOGIC STUDY OF HYPERTENSIVE CEREBROVASCULARITY

高血压脑血管形态学研究

• 批准号: 3401072
• 负责人: DIXON M MOODY
• 金额: $ 16.76万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-04-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3401072
• 关键词: Pongidae; basal ganglia; cerebral aneurysm; cerebrovascular disorders; cerebrovascular occlusions; histochemistry /cytochemistry; human tissue; hypertension; image processing; infarct; laboratory rat; medulla oblongata; mesencephalon; microcirculation; spinal cord

We propose to use the higher resolving power and high contrast of an advanced state-of-the art small focal spot "soft" (low kvp) x-ray unit to study microvascular changes in brains from hypertensive primates and humans and to compare the changes seen with normative controls. Patients with hypertension have a greatly increased incidence of intracerebral hemorrhage and cerebral infarcts. The light microscope has been used extensively to study the microvascular changes in this disease process, but analysis is limited by tissue thickness, i.e., only a small piece if the vascular bed can seen in any one section. Examination of a long, conplex anatomical unit such as an artery-capillary-venule complex is extremely tedious using thin section light microcscope in the evaluation of a longitudinal structure which is to be followed from one plane to the other. Because of the penetrating power and depth of focus, X ray is uniquely capable of examining the vascular bed. Human Material: Specimens of brain tissue from patients with hypertension will be studied for microcirculatory changes from hypertension and compared with normals. Small areas form five specific locations of already fixed brains in addition to spinal cord will be prepared by the histochemical technique originated by the coprincipal investigator and examined by x-ray microscopy. Then these same blocks of tissue will be cut and subjected to a detailed neutopthological examination. Animal Material: Specimens of fixed brain tissue form hypertensive primates will also be prepared, stained by the alkaline phophatase method devised by the coprincipal investigator and examined using both light and x-ray microscopy for microvascular changes and then compared with normals. We will move beyond previous microvascular studies in two areas: a) use of an advanced newly developed, nondestructive testing type x-ray unit that will increase resolving power; b) we will not use capricious barium perfusion angiographic techniques, but will stian the vascular tree directly using a recently developed histochemical staining technique devised by the co-principal investigator. It is believed that this study will identify the premorbid anatomical alteration leading to intracerbral hemorrhage or infarct in patients with hypetension.

我们建议使用更高的分辨率和高对比度 先进的最先进的小焦点“软”（低 kvp）X 射线装置 研究高血压灵长类动物和人类大脑的微血管变化 并将观察到的变化与规范对照进行比较。 患者患有 高血压使脑出血的发生率大大增加 和脑梗塞。 光学显微镜已广泛用于研究微血管 这种疾病过程的变化，但分析受到组织的限制 厚度，即如果在任何一个中都可以看到血管床，则只有一小块 部分。 检查长而复杂的解剖单元，例如 使用薄切片来观察动脉-毛细血管-微静脉复合体是极其繁琐的 光学显微镜用于评估纵向结构 从一架飞机到另一架飞机的跟踪。 因为穿透力 和焦深，X 射线具有独特的能力来检查血管 床。 人体材料：高血压患者脑组织标本 将研究高血压引起的微循环变化并进行比较 与法线。 小区域形成已固定的五个特定位置 除脊髓外，大脑也将通过组织化学制备 技术由副首席研究员发明并通过 X 射线检查 显微镜。 然后这些相同的组织块将被切割并进行 详细的神经病理学检查。 动物材料：高血压脑组织固定标本 灵长类动物也将被制备，通过碱性磷酸酶方法染色 由副首席研究员设计并使用光和光进行检查 X 射线显微镜检查微血管变化，然后与正常情况进行比较。 我们将在两个领域超越以前的微血管研究：a）使用 新开发的先进无损检测型 X 射线装置 将提高分辨能力； b) 我们不会使用反复无常的钡 灌注血管造影技术，但会维持血管树 直接使用最近开发的组织化学染色技术 由联合首席研究员设计。 据信，这项研究将确定病前的解剖学特征 导致患者脑出血或梗塞的改变 高血压。