NEURONAL PATTERNS AND SYNAPSES IN CULTURED COCHLEA

培养耳蜗的神经元模式和突触

• 批准号: 3395936
• 负责人: HANNA M SOBKOWICZ
• 金额: $ 14.34万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1979
• 资助国家: 美国
• 起止时间: 1979-04-01 至 1988-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3395936
• 关键词: afferent nerve; cell differentiation; denervation; ear hair cell; electron microscopy; embryo /fetus tissue /cell culture; innervation; microscopy; nerve endings; organ of Corti; synapses

The overall objective of the proposed research is to comprehend the development of neuronal patterns and differentiation of neuroreceptors in the organ of Corti. The project comprises studies of the development of the isolated organ in culture as compared with that of the intact animal. Of special value is use of the tissue culture technique. We propose the following projects. 1. Ultrastructural study of the developing fetal cochlea in culture. The project is related to development of the innervation pattern in the organ of Corti of the fetal mouse. Three problems will be considered: a) the developmental sequence in the formation of afferent synapses in the fetal organ of Corti, b) the rlation between the differentiation of the hair cell and the acquisition of the synapse, and c) the morphology of the nerve endings of the spiral fibers during the period of innervation-delay of the outer hair cells, a phenomenon especially pronounced in cultures of fetal material. 2. Light-microscope study of the organ of Corti of the Bronx Waltzer mutant mouse in culture. The mutant provides an organ of Corti with a selective population of outer hair cells due to a progressive loss of inner hair cells. The project aims to study the neuronal patterns resulting from the selective loss of inner hair cells. 3. Ultrastructural study of the developing organ of Corti in situ of the Bronx Waltzer mutant mouse to observe the reaction of the afferent synaptic endings and their postsynaptic sites to the degeneration of the receptor cell. 4. Study of the vesiculated nerve endings in culture will be pursued in cultures explanted at different postnatal ages when such endings are known to develop in the intact animal. 5. Study of the patterns of synaptic ribbons in deafferented cultures. Of special interest will be a follow-up of preliminary findings observed in denervated hair cells: a - retaining of synaptic ribbons by the outer hair cells. b - increase in the number of free ribbons. c - formation of reciprocal presynaptic complexes between directly apposed hair cells.

拟议研究的总体目标是理解 神经元模式的发展和神经受体的分化 Corti的器官。 该项目包括有关发展的研究 与完整的动物相比，培养中的孤立器官。 特殊价值是使用组织培养技术。 我们建议 以下项目。 1。发展胎儿的超微结构研究 文化中的耳蜗。 该项目与开发 胎儿皮质器官的神经支配模式。 三 将考虑问题：a） Corti胎儿器官中传入突触的形成，b）rlation 在毛单元的差异和获取之间 突触，c）螺旋纤维神经末端的形态 在外毛细胞的神经支配期间 现象在胎儿物质培养物中尤其明显。 2。 布朗克斯沃尔策突变体的Corti器官的光微镜研究 培养中的小鼠。 该突变体提供了具有选择性的Corti器官 由于内毛的逐渐丧失，外毛细胞的种群 细胞。 该项目旨在研究由 内毛细胞的选择性丧失。 3。超微结构研究 在布朗克斯沃尔策突变小鼠的原位开发器官 观察传入突触结尾的反应及其 突触后的受体细胞变性。 4。研究 文化中将追求文化的囊泡神经末日 当已知这种结局已知时，在不同的产后年龄 在完整的动物中发展。 5。研究突触的模式 脱落文化中的丝带。 特别感兴趣的将是一项后续行动 在不死的毛细胞中观察到的初步发现：a-保留 外毛细胞的突触带。 B-增加数量 免费丝带。 C-形成相互突触前复合物之间的复合物 直接贴上毛细胞。