DEVELOPMENT OF EFFERENT NERVE FIBERS IN THE COCHLEA

耳蜗传出神经纤维的发育

• 批准号: 2125757
• 负责人: HANNA M SOBKOWICZ
• 金额: $ 13.05万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1997-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2125757
• 关键词: DEVELOPMENT; EFFERENT; NERVE; FIBERS; COCHLEA

The long-term objectives of the research are to define the morphological versus immunocytochemical characteristics of the differentiating efferent system in the mouse at the light and electron microscope levels. The isolated organ of Corti in culture will be used as an ultimate control in discerning the innervation provided locally by the spiral ganglion cells from that of central origin. Of special importance will be the study of efferent-like vesiculated endings and their ribbon-afferent/efferent reciprocal synapses with the inner hair cells in the organ of Corti in culture and in the intact animal to determine their local source and immunocytochemical character. The immediate goal is to identify the diversity within the efferent innervation and to determine the developmental sequence of the different subunits. Specifically, we propose: l) to study the detailed developmental pattern of neurofilament immunoreactivity (which labels all neuronal elements) in the intact animal and in culture as a guide for interpreting individual immunocytochemical patterns of cochlear innervation; 2) to study CGRP expression in the innervation of the hair cells and its subsequent extinction from the outer hair cell region; 3) to study immunocytochemically at the electron microscope level the neuroactive substances AChE, ChAT, GAD/GABA, CGRP and enkephalins to determine the sequence of their differentiation and presumptive colocalization; 4) to study the ultrastructural identity of the distinct, new pathway in the inner spiral bundle which retains GAP-43 (growth-associated protein-43) activity in the adult mouse. The most important aspects of the proposed study are first, to discern the neurosensory efferent specializations associated with the inner hair cells presumed to be of cochlear origin; second, to define the ultrastructure and developmental sequence of efferent innervation, and lastly to distinguish between the transitory developmental patterns and their counterparts in the maturing animal. We hope that these studies will transcend species-lines and help us to better understand our own auditory system.

研究的长期目标是定义形态学 与免疫细胞化学特征相对于分化侵出的特征 小鼠在光和电子显微镜水平下的系统。这 培养中孤立的Corti的器官将用作最终控制 辨别螺旋神经节细胞本地提供的神经 从中央起源。特别重要的是研究 传出样的囊泡末端及其带状/带状/传出 与Corti器官中内毛细胞的相互突触 培养和完整的动物，以确定其本地来源 免疫细胞化学特征。直接目标是确定 传出神经内的多样性，并确定 不同亚基的发展序列。具体来说，我们 建议：l）研究神经丝的详细发育模式 完整动物中的免疫反应性（标记所有神经元元素） 在文化中，作为解释个体免疫细胞化学的指南 人工耳蜗神经的模式； 2）研究CGRP表达 毛细胞的神经及其随后从外部灭绝 毛细胞区域； 3）在电子上研究免疫细胞化学 显微镜水平神经活性物质疼痛，聊天，GAD/GABA，CGRP和 Enkephalins确定其分化的顺序和 推定共定位； 4）研究 内部螺旋束中独特的新途径，它保留GAP-43 （与生长相关的蛋白质-43）在成年小鼠中的活性。 拟议研究的最重要方面是首先辨别 与内毛细胞相关的神经感觉传播的专业 假定是人工耳蜗；第二，定义超微结构 和传出神经的发展顺序，最后 区分暂时的发展模式及其 在成熟动物中的对应物。我们希望这些研究能 超越物种线，并帮助我们更好地理解自己的听觉 系统。