FORMATION AND ELIMINATION OF NERVE MUSCLE SYNAPSES

神经肌肉突触的形成和消除

• 批准号: 3395467
• 负责人: JEFFREY L DENBURG
• 金额: $ 12.43万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-04-01 至 1988-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3395467
• 关键词: axon; axon reaction; electrophysiology; histochemistry /cytochemistry; hybridomas; immunochemistry; immunofluorescence technique; innervation; interneurons; membrane activity; monoclonal antibody; motor neurons; muscle; nervous system regeneration; neural transmission; neuroanatomy; neurochemistry; neuromuscular system; nonmammalian vertebrate embryology; synapses; tissue /cell culture

The leg neuromuscular system of the cockroach is one in which the formation of connections between identified motor neurons and muscles may be examined in the adult during axonal regeneration and in the embryo during development. We have demonstrated that during regeneration the coxal depressor muscles are initially randomly innervated by all axotomized neurons. This is similar to what occurs during regeneration in mammals. However, in the cockroach, at later times of regeneration the inappropriate connections are eliminated or the correct ones are maintained until one is left with the original innervation pattern. This is similar to events taking place during embryonic development in mammals. Using anatomical and electrophysiological techniques and surgical manipulations we propose to determine the cellular mechanisms involved in the selective elimination of inappropriate connections. Using various biochemical approaches, including hybridoma technology, we propose to identify, isolate, characterize and demonstrate a function for those macromolecules responsible for generating the specificity of these cellular interactions. In addition, using immunohistochemical techniques, attemps will be made to determine whether the same macromolecules and cellular interactions are involved in the initial formation of the innervation pattern of the muscles during embryonic development. The knowledge acquired in this investigation will help us to attain a better understanding of the more general biological phenomena of intercellular recognition and cell growth. Metastasis of tumors, infection of cells by viruses, bacteria and parasites all involve a specific recognition process. Knowledge of the role of cell surface macromolecules in these processes will lead to better therapeutic treatments. The information acquired on the mechanism of the successful regeneration of cockroach motor neuron may also be applicable to attempts to increase the probability of obtaining successful functional regeneration of neurons after injury in humans. Such knowledge would be helpful for the treatment of paraplegics and for other neurological disorders involving neuronal injury like strokes, multiple sclerosis or head injury.

蟑螂的腿神经肌肉系统是形成的一个 可以检查已鉴定的运动神经元与肌肉之间的连接 在轴突再生期间的成年人和胚胎中 发展。 我们已经证明，在再生期间 抑制剂肌肉最初被所有轴态化而随机支配 神经元。 这类似于哺乳动物再生期间发生的情况。 但是，在蟑螂中，在更新的后期不适当 取消连接或保持正确的连接，直到一个连接 留有原始神经支配模式。 这类似于事件 发生在哺乳动物的胚胎发育期间。 使用解剖学和 我们建议的电生理技术和手术操作 确定选择性消除涉及的细胞机制 不适当的连接。 使用各种生化方法，包括 杂交瘤技术，我们建议识别，隔离，表征和 为那些负责生成的大分子展示功能 这些细胞相互作用的特异性。 另外，使用 免疫组织化学技术，将制定用于确定是否是否 相同的大分子和细胞相互作用涉及 肌肉神经支配模式的初始形成 胚胎发展。 本调查中获得的知识将 帮助我们更好地了解更通用的生物学 细胞间识别和细胞生长的现象。 转移 肿瘤，通过病毒，细菌和寄生虫感染细胞的感染都涉及 具体识别过程。 了解细胞表面的作用 这些过程中的大分子将导致更好的治疗 治疗。 获取有关成功机制的信息 蟑螂运动神经元的再生也可能适用于尝试 增加获得成功功能再生的可能性 人类受伤后的神经元。 这样的知识将有助于 治疗截瘫患者和其他涉及的神经系统疾病 神经元损伤，例如中风，多发性硬化症或头部损伤。