Molecular reporters for measuring proteostasis capacity in cells

用于测量细胞内蛋白质稳态能力的分子记者

• 批准号: DE170100058
• 负责人: A/Prof Yuning Hong
• 金额: $ 26.03万
• 依托单位: La Trobe University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Early Career Researcher Award
• 财政年份: 2017
• 资助国家: 澳大利亚
• 起止时间: 2017-02-01 至 2020-08-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DE170100058
• 关键词: Molecular; reporters; measuring; proteostasis; capacity

This project aims to develop fluorescent dyes to report on the change in unfolded protein load, which reflects the proteostasis status in real time in cells under stress conditions. Proteostasis is a housekeeping process cells undertake to maintain the proper folding and functions of proteins. Perturbation of proteostasis has been linked to neurodegenerative diseases, but chemical probes cannot measure the proteostasis capacity in cells. Intended outcomes include a mechanistic understanding of the relationship between protein misfolding, aggregation and proteostasis. This is expected to ultimately benefit the diagnosis of protein folding diseases, including dementia, and improve the quality of life.

该项目旨在开发荧光染料来报告未折叠蛋白负载的变化，从而实时反映细胞在应激条件下的蛋白质稳态状态。蛋白质稳态是细胞维持蛋白质正确折叠和功能的一个管家过程。蛋白质稳态的扰动与神经退行性疾病有关，但化学探针无法测量细胞中的蛋白质稳态能力。预期结果包括对蛋白质错误折叠、聚集和蛋白质稳态之间关系的机械理解。预计这最终将有利于蛋白质折叠疾病（包括痴呆症）的诊断，并提高生活质量。