Novel ionic-based tools for glycoscience

用于糖科学的新型离子型工具

基本信息

批准号：
EP/J002542/1
负责人：
M. Carmen Galan
金额：
$ 119.24万
依托单位：
University of Bristol
依托单位国家：
英国
项目类别：
Fellowship
财政年份：
2012
资助国家：
英国
起止时间：
2012 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=EP%2FJ002542%2F1
关键词：
Novel ionic based tools glycoscience

项目摘要

Cell surface carbohydrates offer tremendous unexploited potential as molecular fingerprints that can distinguish disease cells from normal cells. However, cell-surface glycans are extraordinarily challenging molecules to characterize, requiring major investments in highly specialized technology and expertise and that is precisely why their full potential has not yet been realized. Developing the tools that will make the study of cell surface carbohydrates possible, offers the possibility of developing early diagnostic tests, as well as new therapeutic targets and vaccines. Despite the importance of these complex molecules, the synthesis of structurally defined complex carbohydrates is still a very laborious process that requires a high level of technical expertise. This lack of rapid, high throughput synthetic methodologies to provide pure products has hampered progress in glycobiology research, which has had to rely on either isolated materials or traditional, lengthy, target-oriented oligosaccharide synthesis. This is a multidisciplinary project involving synthetic organic chemists, glycobiologists, enzymologists and molecular biologists. The proposal centers on the chemical and enzymatic synthesis of novel O-glycan tools following the methodology recently developed within our team, whereby a versatile ionic tag based "catch-and-release" strategy that requires no chromatography after each reaction step, and that is compatible with chemical and enzymatic oligosaccharide synthetic protocols will be employed. The novel oligosaccharide probes will be used for the study of the O-glycosylation patterns of gastrointestinal and ocular mucins that are relevant to disease. We are addressing a timely problem - lack of O-glycan probes for study - while developing the synthetic tools to make oligosaccharide synthesis available to main stream chemists.

细胞表面碳水化合物作为分子指纹提供了巨大的未开发潜力，可以区分疾病细胞和正常细胞。然而，细胞表面聚糖是极难表征的分子，需要对高度专业化的技术和专业知识进行大量投资，这正是其尚未充分发挥潜力的原因。开发使细胞表面碳水化合物研究成为可能的工具，为开发早期诊断测试以及新的治疗靶点和疫苗提供了可能性。尽管这些复杂分子很重要，但结构确定的复杂碳水化合物的合成仍然是一个非常费力的过程，需要高水平的技术专业知识。缺乏快速、高通量的合成方法来提供纯产品，阻碍了糖生物学研究的进展，糖生物学研究不得不依赖于分离的材料或传统的、冗长的、针对目标的寡糖合成。这是一个涉及合成有机化学家、糖生物学家、酶学家和分子生物学家的多学科项目。该提案的重点是新型 O-聚糖工具的化学和酶促合成，遵循我们团队最近开发的方法，即基于多功能离子标签的“捕获和释放”策略，每个反应步骤后不需要层析，即将采用与化学和酶促寡糖合成方案兼容的方法。新型寡糖探针将用于研究与疾病相关的胃肠道和眼部粘蛋白的O-糖基化模式。我们正在解决一个及时的问题 - 缺乏用于研究的 O-聚糖探针 - 同时开发合成工具以使主流化学家可以进行寡糖合成。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Lactose as a "Trojan Horse" for Quantum Dot Cell Transport

乳糖作为量子点细胞运输的“特洛伊木马”

DOI：
10.1002/ange.201307232
发表时间：
2013
期刊：
Angewandte Chemie
影响因子：
0
作者：
Benito-Alifonso D
通讯作者：
Benito-Alifonso D

Stereoselective synthesis of protected l- and d-dideoxysugars and analogues via Prins cyclisations.

DOI：
10.1039/c5sc04144a
发表时间：
2016-04-21
期刊：
Chemical science
影响因子：
8.4
作者：
Beattie RJ;Hornsby TW;Craig G;Galan MC;Willis CL
通讯作者：
Willis CL

Lactose as a "Trojan horse" for quantum dot cell transport.

DOI：
10.1002/anie.201307232
发表时间：
2014-01-13
期刊：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
影响因子：
16.6
作者：
Benito-Alifonso, David;Tremel, Shirley;Hou, Bo;Lockyear, Harriet;Mantell, Judith;Fermin, David J.;Verkade, Paul;Berry, Monica;Galan, M. Carmen
通讯作者：
Galan, M. Carmen

Platform Synthetic Lectins for Divalent Carbohydrate Recognition in Water.

DOI：
10.1002/anie.201603082
发表时间：
2016-08-01
期刊：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
影响因子：
16.6
作者：
Carter, Tom S.;Mooibroek, Tiddo J.;Stewart, Patrick F. N.;Crump, Matthew P.;Galan, M. Carmen;Davis, Anthony P.
通讯作者：
Davis, Anthony P.

Synthesis of mucin-type O-glycan probes as aminopropyl glycosides.