SYMBOLIC SIMULATION OF DNA METABOLISM

DNA 代谢的符号模拟

基本信息

批准号：
3374064
负责人：
DOUGLAS L BRUTLAG
金额：
$ 20.33万
依托单位：
STANFORD UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-07-01 至 1992-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3374064
关键词：
DNA artificial intelligence chemical models computer simulation decision making enzyme mechanism enzyme substrate nucleic acid metabolism

项目摘要

Using established methods of knowledge representation and rule- based expert systems I propose to develop a symbolic simulation of DNA metabolism. Rule-based expert systems permit simulations of DNA metabolism which can predict the activity of both individual enzymes and of entire pathways under a wide variety of physiological conditions. Both classical production rules and an assumption-based truth maintenance system will be used. Rule-based methods have the intrinsic property of being able to explain conclusions using English sentences or graphic representations of the pattern of inference. The enzymes, substrates and products will also be represented in a frame-based knowledge system. Knowledge based systems facilitate the description of product-precursor relationships between different metabolites as well as the complete pathway. Physiological conditions such as the presence of salts, nucleotides, temperature, ionic strength, pH, etc. will be presented graphically as active images of thermometers, gauges and switches. All environmental and substrate conditions can be changed by using a mouse pointing device. In response to changes of any parameter, a series of rules defining the specificity and reactivity of enzymes are automatically invoked and specific conclusions are made. The methods for specifying the actions of enzymes are well defined, allowing ready simulation of other enzymes. Simulations of individual enzymatic steps can be coupled together to generate a discrete event simulation of the entire metabolic pathway.

使用知识表示和规则的既定方法 - 我建议开发符号模拟的基于的专家系统 DNA代谢。基于规则的专家系统许可证 DNA代谢的模拟可以预测单个酶和宽阔的整个途径各种生理条件。两者都是古典作品规则和基于假设的真实维护系统将是用过的。基于规则的方法具有存在的内在属性能够使用英语句子或图形来解释结论推理模式的表示。酶，底物和产品也将在基于框架的知识系统。基于知识的系统促进产品 - 前身关系的描述不同代谢物以及完整的途径。生理条件，例如盐，核苷酸的存在，温度，离子强度，pH等将以图形方式显示作为温度计，量表和开关的主动图像。全部可以通过使用一个鼠标指向设备。响应任何参数的变化，定义酶的特异性和反应性的一系列规则自动调用并得出具体的结论。指定酶作用的方法很好定义，可以准备好对其他酶的模拟。模拟可以将单个酶促步骤耦合在一起以生成整个代谢途径的离散事件模拟。