CARDIOVASCULAR DETERMINANTS OF CATECHOLAMINE RELEASE

儿茶酚胺释放的心血管决定因素

基本信息

批准号：
3335719
负责人：
GORDON L KAUFFMAN
金额：
$ 23.38万
依托单位：
PENNSYLVANIA STATE UNIV HERSHEY MED CTR
依托单位国家：
美国
项目类别：
财政年份：
1977
资助国家：
美国
起止时间：
1977-09-01 至 1992-11-30
项目状态：
已结题

项目摘要

Evaluation of the role of Prostaglandin E2 (PGE2) delivered systemically as intravenously infused PGE2 and centrally by ventriculo-cisternal perfusion in controlling centrally mediated, Angiotensin II dependent, reflexly excited adrenal medullary catecholamine release in acutely anephric dogs are our goals for the next grant period. In anesthetized, heparinized dogs following acute nephrectomy and placement of adreno-femoral shunts and aortic cannulae we will study the dose responsiveness of intravenous PGE2's ability to restore normal adrenal reflex catecholamine release elicited by acute hemorrhage. With an appropriate sub-systemic threshold dose of PGE2 derived from that data, we then will perfuse ventriculo-cisternal PGE2 in anesthetized acutely anephric dogs with threshold quantities of PGE2 on reflex catecholamine release following hemorrhage. Finally, we will infuse, ventriculo-cisternally, Angiotensin II and PGE2 concomitantly to define the central effect of PGE2 on Angiotensin II responsiveness. Ventriculo-cisternally perfused PGE2 will be evaluated in two different ways; in one set of experiments, I.V. Angiotensin and ventriculo-cisternally perfused PGE2 will both be given and in another set of experiments, PGE2 and Angiotensin II will be given concominantly in the ventriculo-cisternal perfusion circuit. Understanding the neuro-hormonal control reflex catecholamine release is one long-term objective; another related goal is understanding whether or not renal hormones, specifically renin and the Angiotensin II generated under its infuence and PGE2, are needed to support central nervous system reflex activity. Such physiologic insights will, we hope, help us understand disordered central nervous system mechanisms involved in clinical hypertension.

评估前列腺素E2（PGE2）的作用，以系统地交付静脉注入PGE2，并由心室灌注中心在控制集中介导的血管紧张素II依赖性，反射激发的肾上腺髓样儿茶酚胺在急性Anephric狗中释放是我们下一个赠款时期的目标。用麻醉，肝素化的狗急性肾切除术和adreno-Femoral分流的放置和主动脉插管我们将研究静脉内PGE2的剂量反应性能够恢复正常肾上腺反射儿茶酚胺释放的能力急性出血。 PGE2的适当子系系统阈值剂量然后，我们将从该数据中得出，然后将脑室室中的pge2在麻醉的急性抗狗，阈值量的PGE2量出血后反射儿茶酚胺释放。最后，我们会的注入，室心形，血管紧张素II和PGE2同时进行定义PGE2对血管紧张素II反应性的核心作用。将在两种不同方式在一组实验中，i.v.血管紧张素和将给出脑室灌注PGE2，并在另一组中给出在实验中，PGE2和血管紧张素II将同时给出心室灌注回路。了解神经激素控制反射儿茶酚胺释放是一个长期目标。其他相关目标是了解肾荷尔蒙是否特别是肾素和血管紧张素II在其Inforence和pge2下产生，为需要支持中枢神经系统反射活动。这样的生理我们希望，洞察力将帮助我们理解无序的中枢神经涉及临床高血压的系统机制。