COMPARATIVE STUDY OF COAGULATION AND VASCULAR DISEASE

凝血与血管疾病的比较研究

• 批准号: 3334317
• 负责人: WINIFRED J DODDS
• 金额: $ 21.03万
• 依托单位: NYSDOH/HEALTH RESEARCH, INC.
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3334317
• 关键词: animal colony; animal population genetics; binding proteins; biological signal transduction; blood coagulation disorders; blood coagulation tests; calcium metabolism; cats; coagulation factor VIII; congenital blood disorder; cow; cyclic AMP; disease /disorder model; dogs; enzyme linked immunosorbent assay; fibrinogen; fluorescent dye /probe; gel electrophoresis; genetic models; goats; guinea pigs; hamsters; hemophilia As; hemorrhagic disorders; hemostasis; horses; human tissue; hypothyroidism; immunochemistry; immunoelectrophoresis; ionophores; laboratory mouse; laboratory rabbit; laboratory rat; membrane activity; phosphodiesterase inhibitors; phosphodiesterases; phospholipids; phosphorylation; platelet aggregation; platelet disorder; platelets; sheep; thrombocytopathy; thrombosis; von Willebrand's disease

Colonies of animals with inherited coagulation and platelet function defects are maintained for genetic, biochemical and physiological studies; production of reagents for coagulation tests; and evaluation of hemostasis. These include dogs with hemophilia A, hemophilia B, von Willebrand's disease (VWD), factor VII deficiency, warfarin hypersensitivity, thrombasthenic thrombopathia, thrombopathia and various combinations of these defects,; fawn-hooded rats with platelet storage pool disease, spontaneous glomerulosclerosis and hypertension; and Syrian hamsters with age-associated spontaneous atrial thrombosis. Studies with these naturally occurring animal models examine basic mechanisms of hemostasis and thrombosis in comparison to analogous human diseases. The objectives for the next period include continuing to diagnose, characterize, maintain and research animal models of spontaneous hemorrhagic and thrombotic diseases; studies by in vitro and in vivo methods of factor VIII in normal, hemophilic and VWD dogs; studies of the platelet function defect of basset hounds; collaboration with other investigators in research projects; provision of reagents, genetic material and other animal products based on their availability; consultation about the comparative aspects of hemostasis research and bleeding disorders; ad provision of space for visiting scientists or laboratory personnel. Specific aims for the period include: refinement of a multimeric assay for canine von Willebrand's factor (VWF) for diagnostic and biochemical studies; research with rDNA-derived human factor VIII in hemophilic and VWD dogs; studies of the molecular and functional properties of canine VWF and factor VIII coagulant in thyroid disease; and platelet studies focused on definition of the biochemical defect of canine thrombopathia, specifically its abnormal signal-response coupling via the cyclic nucleotide, phosphodiesterase and calmodulin pathways. Screening tests for thrombopathia using whole blood and platelet agonists and the snake-venom-induced agglutination assay for canine plasma VWF are used in the field for diagnostic purposes.

具有遗传凝结和血小板的动物菌落 维持功能缺陷，以用于遗传，生化和 生理研究；生产用于凝血测试的试剂； 和止血的评估。 这些包括血友病的狗 A，血友病B，冯·威勒布兰氏病（VWD），因子VII 不足，华法林超敏反应，血栓性 血小板病，血小板病和各种组合 缺陷；小鹿的大鼠患有血小板存储池疾病， 自发性肾小球硬化和高血压；和叙利亚 与年龄相关的自发性心房血栓形成的仓鼠。 这些自然发生的动物模型的研究检查 与 类似的人类疾病。 下一个时期的目标包括继续诊断， 自发的特征，维护和研究动物模型 出血和血栓性疾病；体外和IN的研究 正常，血友病和VWD犬中因子VIII的体内方法； 贝塞猎犬的血小板功能缺陷的研究； 与其他研究人员在研究项目中的合作； 提供试剂，遗传物质和其他动物产品 根据它们的可用性；关于比较的咨询 止血研究和出血疾病的各个方面；广告 为来访的科学家或实验室人员提供空间。 该期间的具体目的包括：多聚体的完善 犬Von Willebrand因子（VWF）的测定法和 生化研究； rDNA衍生的人类因素的研究 VIII在血友病和VWD犬中；分子和 犬VWF和因子VIII凝结剂的功能特性 甲状腺疾病；和血小板研究的重点是 犬类血小板症的生化缺陷，特别是它的生化缺陷 通过环状核苷酸，异常信号反应耦合， 磷酸二酯酶和钙调蛋白途径。 筛选测试 血小板病使用全血和血小板激动剂，以及 蛇血浆血浆VWF的蛇诱导的凝集测定法 用于诊断目的。