NUCLEO-CYTOPLASMIC INTERACTIONS IN EGGS AND OOCYTES

卵子和卵母细胞中的核质相互作用

• 批准号: 3316224
• 负责人: DAVID J NISHIOKA
• 金额: $ 5.2万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1989-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3316224
• 关键词: Asteroidea; autoradiography; cell fusion; cell growth regulation; cell nucleus; cytoplasm; egg /ovum; embryogenic cleavage; enzyme inhibitors; fertilization; nonmammalian vertebrate embryology; phase contrast microscopy; saltwater environment; sea urchins; systematic biology

The control of the cell cycle in early sea urchin and starfish embryos will be studied in terms of nucleo-cytoplasmic interactions. Aphidicolin, a recently discovered drug which inhibits DNA replication specifically, will be applied to early embryos in connection with the recently developed technique of egg fusion. This experimental approach is designed to make several new determinations about cell cycle control in the early embryo: (1) whether or not the unreplicated nucleus exerts negative control over progression through the cell cycle; (2) whether or not the cytoplasm of the replicated cell can exert positive control over the unreplicated nucleus; and (3) whether or not any controlling factors inferred from these studies can cross taxonomic class barriers between sea urchins and starfish. Additional new determinations about necleocytoplasmic interactions in unfertilized eggs and immature oocytes using the egg fusion technique only include: (4) whether or not nuclear DNA replication is stimulated in an unfertilized sea urchin egg upon fusion with a fertilized egg; (5) whether or not germinal vesicle breakdown and maturation of an immature sea urchin oocyte is stimulated upon fusion with an unfertilized egg; (6) whether or not germinal vesicle breakdown and maturation of an immature starfish oocyte is stimulated upon fusion with a mature starfish oocyte; and (7) whether or not any controlling factors inferred from these studies can cross taxonomic class barriers between sea urchins and starfish. These determinations should provide a more complete understanding of how the nucleus and cytoplasm are related with one another during the production of the egg and through early cleavage of the embryo.

早期海胆和海星胚胎细胞周期的控制将 可以从核质相互作用的角度进行研究。 阿菲迪霉素，一种 最近发现的药物可以特异性抑制DNA复制， 应用于与最近开发的相关的早期胚胎 鸡蛋融合技术。 这种实验方法的目的是使 关于早期胚胎细胞周期控制的几个新决定： (1)未复制核是否对复制体发挥负控制作用 细胞周期的进展； (2) 细胞质是否 复制的细胞可以对未复制的细胞核进行正控制； (3) 从这些研究中是否推断出任何控制因素 可以跨越海胆和海星之间的分类学界限。 关于核细胞质相互作用的其他新测定 仅使用卵子融合技术的未受精卵和未成熟卵母细胞 包括：（4）细胞核DNA复制是否被刺激 未受精的海胆卵与受精卵融合； (5) 是否 或未成熟海胆的生泡破裂和成熟 卵母细胞与未受精卵融合后受到刺激； (6) 是否或 不是未成熟海星的生发囊泡分解和成熟 卵母细胞与成熟海星卵母细胞融合后受到刺激；和（7） 从这些研究中推断出的任何控制因素是否可以 跨越海胆和海星之间的分类学界限。 这些 的决定应提供对如何 细胞核和细胞质在生成过程中相互关联 卵子和胚胎的早期分裂。