FUNCTION OF THE DECIDUAL TISSUE

蜕膜组织的功能

• 批准号: 3311878
• 负责人: GEULA GIBORI
• 金额: $ 12.96万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-12-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311878
• 关键词: SDS polyacrylamide gel electrophoresis; angiogenesis; apoptosis; cell death; cell differentiation; decidua; drug delivery systems; endometrium; endonuclease; estradiol; fibroblast growth factor; follicle stimulating hormone; gene expression; hormone inhibitor; hormone regulation /control mechanism; in situ hybridization; laboratory rat; messenger RNA; mixed tissue /cell culture; nucleoproteins; ovary; pregnancy; progesterone; progesterone receptors; prolactin; pseudopregnancy; reproductive hormone; tissue /cell culture; transforming growth factors

The overall goal of this project is to examine the control and the role of decidual tissue in the maintenance of pregnancy in the rat. The first aim will determine whether bFGF is responsible for the intense angiogenesis that takes place in the site of nidation and decidualization, whether it is produced by either perivascular decidual cells and/or endothelial cells, and whether its synthesis is controlled by ovarian steroids. A morphological hallmark of decidualization is a striking transformation of stromal cells into two different cell populations localized in the mesometrial and antimesometrial region of the uterus. Since both TGFalpha and EGF receptors, through which TGFalpha stimulates cell differentiation, are expressed in the decidual tissue, experiments are proposed to determine whether: 1) differentiation and possibly cell proliferation of endometrial stromal cells are induced by TGFalpha, and whether 2) immunoneutralization of TGFalpha can prevent the action of both exogenously administered TGFalpha on nontransformed cells and the endogenously formed growth factor in transformed cells. The possibility that either TGFalpha and/or EGF receptor expression is regulated by ovarian steroids, which are prerequisites for decidualization, will be examined. Because stromal cell differentiation occurs first in the antimesometrial region of the uterus, and only several days later in the mesometrial region, the temporal expression of TGFalpha and EGF receptors in both tissues will be assessed throughout the development of the decidua. The second aim of this proposal will test the hypothesis that decidual tissue regression is due to a programmed form of cell death or apoptosis, more specifically, 1) whether cell death is accompanied by DNA fragmentation and increased endonuclease activity, 2) whether new genes encoding endonucleases are expressed during decidual cell regression, and 3) whether progesterone and progesterone receptors play a role in decidual programmed cell death. The third aim of this proposal is to gain further insight into the secretion and action of decidual hormone. This will examine the physiological role of decidual follistation, a FSH-inhibitor expressed abundantly in the decidua, on follicular secretion of estradiol, and that of decidual luteotropin on mesometrial expression of alpha2 macroglobulin. Alpha2 macroglobulin is a potent protease inhibitor which appears to play an important role in limiting trophoblast cell invasion. The last specific aim proposes to establish a stable cell line of functional mesometrial and antimesometrial cells in order to reduce the number of animals used, and to obtain an unlimited number of hormone-secreting cells, either mesometrial or antimesometrial.

该项目的总体目标是检查控制和作用 在大鼠维持妊娠中的判决组织。 第一个目标 将确定BFGF是否负责强烈的血管生成 这发生在麻烦和deDIADIALIAD的站点中，是否是 由血管周期细胞和/或内皮细胞产生， 以及它的合成是否由卵巢类固醇控制。 一个 义词化的形态学标志是一个惊人的转变 基质细胞成两个不同的细胞种群 子宫的介质和脉络膜分析区域。 由于两个TGFALPHA 和EGF受体，TGFALPHA刺激细胞分化， 在决策组织中表达，提出了实验以确定 是否：1）分化和可能是子宫内膜的细胞增殖 基质细胞由TGFALPHA诱导，是否2）免疫中性化 tgfalpha可以防止两者的作用 在非转化细胞上的TGFALPHA和内源形成的生长因子 在转化的细胞中。 TGFALPHA和/或EGF的可能性 受体表达受卵巢类固醇的调节， 将检查义词化的先决条件。 因为基质细胞 分化首先发生在子宫的抗时符区域中， 仅几天后，在介质区域（时间） 将评估两种组织中TGFALPHA和EGF受体的表达 在整个Decidua的整个发展中。 该提议的第二个目标 将检验以下假设，即决定性组织回归是由于 细胞死亡或凋亡的编程形式，更具体地说，1） 细胞死亡伴随着DNA碎片和核酸内切酶增加 活动，2）是否表达了编码内切核酸酶的新基因 决定性细胞回归，3）孕酮和孕激素是否 受体在判决编程的细胞死亡中起作用。 第三个目标 该提议是为了进一步了解 决荷。 这将检查决定的生理作用 Follistation是一种在Decidua中表达的FSH抑制剂， 雌二醇的卵泡分泌，以及deciDual黄质蛋白的分泌 α2大球蛋白的介质表达。 alpha2巨球蛋白是 有效的蛋白酶抑制剂似乎在 限制滋养细胞侵袭。 最后一个特定目的提议 建立稳定的功能性介质和抗时符的细胞系 为了减少所使用的动物数量，并获得 无限数量的激素分泌细胞，无论介质或 脉冲假体。