'Horticulture' CRISPR Cas-mediated and inter-species transfer of broad-spectrum, potentially durable disease resistance in crop plants (CRIMIST-DR).

“园艺”作物中 CRISPR Cas 介导的广谱、潜在持久抗病性的种间转移 (CRIMIST-DR)。

• 批准号: BB/X011798/1
• 负责人: John Walsh
• 金额: $ 6.42万
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FX011798%2F1
• 关键词: Horticulture; CRISPR; Cas; mediated; inter

The virus called turnip mosaic virus (TuMV) is an important pathogen infecting many crop plant types, reducing yields and making them unmarketable. In order to reproduce, the virus has to use certain proteins in plants. Without these proteins it cannot reproduce. One such plant protein is called the eukaryotic initiation factor iso4E [eIF(iso)4E]. We have shown that in Chinese cabbage (Brassica rapa sub-species pekinensis) which has three copies of eIF(iso)4E, if one of the copies is missing, the virus cannot reproduce, but the plant is unaffected. Chinese cabbage varieties with this resistance (that was identified and characterised at the University of Warwick) are currently being developed by an international seed company (Syngenta). Chinese cabbage is the most important vegetable brassica crop worldwide.We have been unable to identify any resistance to TuMV in the related and important plant species Brassica oleracea, which includes broccoli, cabbage, cauliflower, kale, Brussels sprouts etc. In collaboration between the Elizabeth Creak Horticultural Technology Centre and the Plant Virology Group at the University of Warwick, NIAB and the commercial plant breeding company Syngenta, we aim to knock out the copy of eIF(iso)4E that TuMV needs in order to reproduce in B. oleracea utilising gene editing technology, thereby establishing a technique to rapidly develop virus-resistant varieties of the different B. oleracea types.In collaboration with the commercial seed company Syngenta, we also aim to move the copy of eIF(iso)4E that the virus cannot use from Chinese cabbage into B. oleracea by conventional crossing, in order to develop virus-resistant plants by this alternative route.

萝卜花叶病毒 (TuMV) 是一种重要的病原体，可感染多种农作物，导致产量下降并无法销售。为了繁殖，病毒必须使用植物中的某些蛋白质。没有这些蛋白质，它就无法繁殖。一种这样的植物蛋白称为真核起始因子 iso4E [eIF(iso)4E]。我们已经证明，大白菜（Brassica rapa sub-species pekinensis）具有三个 eIF(iso)4E 拷贝，如果其中一个拷贝缺失，病毒就无法繁殖，但植物不受影响。具有这种抗性的大白菜品种（由华威大学鉴定和鉴定）目前正在由一家国际种子公司（先正达）开发。大白菜是世界上最重要的蔬菜芸苔属作物。我们无法在相关的重要植物品种甘蓝中发现任何对 TuMV 的抗性，其中包括西兰花、卷心菜、花椰菜、羽衣甘蓝、球芽甘蓝等。 Creak 园艺技术中心和华威大学植物病毒学小组、NIAB 和商业植物育种公司先正达，我们的目标是淘汰复制品TuMV 利用基因编辑技术在甘蓝中繁殖所需的 eIF(iso)4E，从而建立了一种快速开发不同甘蓝类型的抗病毒品种的技术。与商业种子公司先正达合作，我们他们还旨在通过常规杂交将病毒无法利用的 eIF(iso)4E 副本从大白菜转移到甘蓝中，从而通过这种替代途径培育出抗病毒植物。