STRUCTURE & FUNCTION OF THE EPIDIDYMIS AND VAS DEFERENS

结构

• 批准号: 3311737
• 负责人: DAVID W HAMILTON
• 金额: $ 16.93万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-09-29 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3311737
• 关键词: O glycosidase; Sertoli cells; antimetabolites; blood proteins; chorionic gonadotropin; corticosterone; epididymis; epithelium; estradiol; follicle stimulating hormone; galactosyltransferases; gel electrophoresis; genital secretion; glycoproteins; glycosylation; hormone regulation /control mechanism; immunoelectrophoresis; isolation perfusion; laboratory rat; lactalbumin; lectin; membrane activity; membrane proteins; organelles; pituitary gonadal axis; prolactin; protein biosynthesis; radiotracer; secretion; sperm; sperm motility; testosterone

The experiments described in this application address the overall question of how proteins and glycoproteins attach to sperm surfaces as sperm proceed through the epididymis. The importance to health of the questions being asked is that as sperm enter the epididymis they are unable to fertilize eggs, but after moving through the epididymis they are. Thus, understanding "maturational" events (such as how molecules from epididymal fluid attach to sperm) will help in the genera understanding of the mechanisms both of fertility and infertility. We have discovered that a approximately 24 kDa antigen on rat sperm plasma membrane, which is added to sperm in the epididymis, is linked to the sperm plasma membrane by way of phosphotidylinositol (PI), a new linkage that has been found in a number of important cell surface molecules. It is proposed in this application to study in detail the linkage approximately 24 kDa has with sperm, to purify approximately 24 kDa and to do amino acid composition and sequence studies. It is also proposed to identify the site of synthesis of precursor(s) approximately 24 kDa and the mechanisms regulating its (their) synthesis (using cDNA and cRNA probes, as well as precursor incorporation) and secretion. The grant will also address the mechanisms of the sequence of binding of approximately 24 kDa to sperm. Finally, it is proposed to search for a physiological role for approximately 24 kDa, particularly related to motility and energy substrate transport.

本申请地址中描述的实验总体 蛋白质和糖蛋白如何附着于精子表面的问题 随着精子的进行，经过附加膜。 对健康的重要性 所问的问题是，当精子进入附加膜时 他们无法施肥，但是在穿过 它们是附子。 因此，了解“成熟”事件 （例如，附子流体的分子如何附着在精子上） 帮助属理解机制的属 生育和不育。 我们发现大鼠精子上约有24 kDa抗原 质膜，在附睾中添加到精子中，是 通过磷酸丁乙醇链接到精子质膜 （pi），在许多重要的地方发现的新链接 细胞表面分子。 在此申请中提出了 详细研究大约24 kDa与精子的联系， 净化大约24 kDa并进行氨基酸组成和 序列研究。 还建议确定 前体的合成约24 kDa和机制 调节其（其）合成（使用cDNA和CRNA探针作为 以及前体掺入）和分泌。 赠款意志 还解决了结合序列的机制 大约24 kDa到精子。 最后，建议搜索 对于大约24 kDa的生理作用，特别是 与运动性和能量底物传输有关。