STRUCTURE & FUNCTION OF THE EPIDIDYMIS AND VAS DEFERENS

结构

基本信息

批准号：
3311733
负责人：
DAVID W HAMILTON
金额：
$ 10.94万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
1978
资助国家：
美国
起止时间：
1978-09-29 至 1988-03-31
项目状态：
已结题

项目摘要

The primary goals of this application are to elucidate mechanisms of maturation of spermatozoa in the epididymis which will lead to an understanding of how fertility is regulated in the male. Maturation of sperm in the epididymis involves interactions among sperm, luminal fluid, and the epididymal epithelium (which is regulated by factors from the blood vascular system). In the experiments to be done in this application, investigation will be undertaken on proteins which are synthesized and secreted by the epididymal epithelium. Antibodies raised against these proteins cross-react with molecules on the sperm surface as well as with molecules that are derived from the testis. The first aim of this grant proposal is to isolate pure compounds from rete testis fluid, from the epididymis, and from sperm surfaces, which will then be studied extensively with respect to their amino acid sequences and the carbohydrate components of their oligosaccharide chains. The underlying theme of these experiments is to determine whether all of the molecules that cross-react immunologically are actually structurally the same, and whether they are the same as the Alpha-lactalbumin from rat milk. After deducing their structure, the molecules will be studied with respect to their ability to modify galactosyltransferase activity, which is a major function of Alpha-lactalbumin. The third aim in the project is related to studies on the synthesis and secretion (and control of these two functions) of immunologically cross-reactive molecules from the epididymis in culture. We have developed a perifusion culture system for epididymal tubules that will allow us to do studies on long-term cultures of epididymis, in which we know that epididymal epithelium and sperm are reasonably normal. Finally, using the well characterized monoclonal antibodies raised against the individual polypeptides of the epididymal Alpha-lactalbumin complex, experiments will be done to determine whether the molecules have any effect on sperm motility or fertilizing ability.

该应用的主要目标是阐明附睾中精子的成熟，这将导致了解男性如何调节生育能力。成熟附睾中的精子涉及精子，腔内流体之间的相互作用，和附子上皮（由血液的因素调节血管系统）。在此应用程序中要完成的实验中，调查将对合成的蛋白质进行，并由附睾上皮分泌。提出的抗体蛋白质与精子表面上的分子交叉反应以及源自睾丸的分子。这笔赠款的第一个目的建议是将纯化合物与rete睾丸液分离附睾和精子表面，然后将广泛研究关于其氨基酸序列和碳水化合物成分它们的寡糖链。这些实验的基本主题是确定是否所有交叉反应的分子在结构上，免疫学上实际上是相同的，它们是否是与大鼠牛奶中的α-乳脂蛋白相同。推论之后结构，将研究分子的能力修改半乳糖基转移酶活性，这是 α-乳蛋白。该项目的第三目与研究有关的合成和分泌（以及对这两个功能的控制）来自附睾中培养的免疫交叉反应分子。我们已经开发了一种针对附睾小管的渗出培养系统我们将允许我们对附睾长期培养的研究进行研究，其中我们知道附子上皮和精子是合理正常的。最后，使用具有良好特征的单克隆抗体附睾α-乳蛋白蛋白复合物的单个多肽，将进行实验以确定分子是否有任何作用关于精子运动或受精能力。