BACULOVIRUS INDUCED NUCLEAR MEMBRANE ASSEMBLY

杆状病毒诱导的核膜组装

• 批准号: 3307078
• 负责人: MAX D SUMMERS
• 金额: $ 17.21万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3307078
• 关键词: Baculoviridae; SDS polyacrylamide gel electrophoresis; antiserum; cell cycle proteins; cell membrane; cell nucleus; cytoplasm; gene expression; genetic mapping; genetic markers; glycoprotein biosynthesis; glycosylation; intracellular transport; laboratory mouse; laboratory rabbit; membrane activity; membrane proteins; molecular genetics; nucleic acid sequence; nucleocapsid; polymerase chain reaction; posttranslational modifications; protein structure function; site directed mutagenesis; transport proteins; ubiquitin; virus genetics; virus replication

Baculoviruses are enveloped nucleocapsids which mature both in the nucleus and at the cell surface. The acquisition of an envelope in the cell nucleus requires the synthesis or transport of large quantities of viral envelope substrates to the nucleoplasm. The extent of synthesis and nature of budding of the baculovirus nucleocapsid in the nucleus is a unique phenomenon compared to other virus envelope maturation strategies. Comparison of the structural proteins of the nuclear and cytoplasmic viral forms show major differences in number, size, and in glycosylation and phosphorylation modifications. Studies of the viral genes and their encoded proteins which are unique to either viral form should provide important insights on the molecular basis of viral maturation in the nucleus. The studies proposed are: (a) to map and sequence envelope and nucleocapsid genes that encode proteins that reflect characteristics specific to each form of the baculovirus Autographa californica and which show potential for use as markers; (b) to use these characteristics to study expression, regulation of expression, processing, trafficking and assembly in infected cells. Emphasis will be on the origin and assembly of viral envelopes and the viral maturation process in the nucleus.

杆状病毒是包膜的核蛋白酶，都在成熟 核和细胞表面。 在 细胞核需要合成或运输大量 病毒包膜底物到核质。 合成的程度 细胞核中杆菌病毒核蛋白酶芽孢杆菌的发芽性质和 与其他病毒包膜成熟策略相比，独特的现象。 比较核和细胞质的结构蛋白 病毒形式在数量，大小和糖基化方面显示主要差异 和磷酸化修饰。 对病毒基因及其编码蛋白的研究 任何一种病毒形式都应对分子提供重要的见解 核中病毒成熟的基础。 提出的研究是：（a） 映射和序列包膜和核素基因，编码蛋白质 这反映了特定于杆状病毒的每种形式的特征 加利福尼亚的Autographa，这显示出有用作标记的潜力； （b） 要使用这些特征来研究表达，请调节 感染细胞中的表达，加工，运输和组装。 重点将放在病毒信封的起源和组装上 细胞核中的病毒成熟过程。