BACULOVIRUS INDUCED NUCLEAR MEMBRANE ASSEMBLY

杆状病毒诱导的核膜组装

• 批准号: 2701569
• 负责人: MAX D SUMMERS
• 金额: $ 22.1万
• 依托单位: TEXAS A&M AGRILIFE RESEARCH
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-05-01 至 2000-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2701569
• 关键词: Baculoviridae; gene expression; gene mutation; laboratory mouse; laboratory rabbit; membrane biogenesis; nuclear membrane; polymerase chain reaction; posttranslational modifications; protein transport; recombinant proteins; site directed mutagenesis; structural genes; virus antigen; virus assembly; virus envelope; virus genetics; virus protein

A novel characteristic of baculovirus infection is the unusual and abundant induction, synthesis and assembly of cellular membranes, particularly in the nucleoplasm. The molecular strategy utilized by the baculovirus to obtain an envelope is quite unique. De novo membrane assembly in the nucleoplasm has been hypothesized as the process for the elaborate induction of intranuclear membranes; however, this theory is not consistent with current models of eukaryotic cell membrane assembly and lipid biosynthesis. Our studies identify several PDV envelope proteins and one of these, PDV-E66, is present in intranuclear microvesicles and the viral envelope. This is the first biochemical evidence that the nuclear microvesicles function as a source or an intermediate of the PDV viral envelope. The identification of PDV envelope proteins provide markers to allow the biosynthesis, trafficking and assembly of membranes which occur in the infected cell nucleoplasm. Our observations and preliminary data suggest that the Autographa californica nuclear polyhidrosis baculovirus may encode homologs of key cellular proteins that may function to influence inner nuclear membrane activities and de novo lipid synthesis. This study may not only reveal the novel envelopment strategies utilized by AcMNPV but could also reveal new details of eukaryotic cellular pathways of transport and nuclear membrane biogenesis. AcMNPV has become the most extensively studied baculovirus because of its use as a cloning and expression vector and its use and development for crop protection as a natural or genetically engineered microbial pest control agent. The biology of baculovirus-host interactions is uniquely complex yet studies of this virus-hose system are serving an important role in the development of fundamental knowledge of molecular and biochemical pathways in insects and invertebrates for comparison with the more comprehensively studied vertebrate systems. These studies are relevant to the very significant role that invertebrates play in the transmission of disease to plants, animals and humans and for their use in pest control strategies fundamental to crop protection and public health.

杆状病毒感染的新型特征是不寻常的， 细胞膜的丰富诱导，合成和组装， 特别是在核质中。 由 获得信封的细菌病毒是非常独特的。 从头膜 核质中的组装已被认为是 精心诱导核内膜；但是，这个理论不是 与当前真核细胞膜组装的模型一致 脂质生物合成。 我们的研究确定了几种PDV包膜蛋白 其中之一是PDV-E66，存在于核内微泡中， 病毒信封。 这是第一个生化证据 核微囊泡作为PDV的来源或中间体的作用 病毒信封。 PDV包膜蛋白的鉴定提供 标记允许膜的生物合成，贩运和组装 发生在感染的细胞核质中。 我们的观察和 初步数据表明加州核心核核 多角病杆状病毒可能编码关键细胞蛋白的同源物 可能会影响内部核膜活性和从头开始 脂质合成。 这项研究不仅可能揭示了新颖的信封 ACMNPV使用的策略，但也可以揭示 运输和核膜生物发生的真核细胞途径。 ACMNPV已成为最广泛研究的细菌病毒，因为它 用作克隆和表达向量及其使用和开发 作物保护是天然或基因工程的微生物害虫 控制代理。 杆状病毒宿主相互作用的生物学是独特的 对该病毒软管系统的复杂研究正在为重要 在发展基本知识和分子知识中的作用 昆虫和无脊椎动物的生化途径与 更全面地研究了脊椎动物系统。 这些研究是 与无脊椎动物在 向植物，动物和人类传播疾病，并用于使用 害虫控制策略是农作物保护和公共卫生的基础。