Protein disorder in crop stress adaptation

作物逆境适应中的蛋白质紊乱

• 批准号: BB/Z514986/1
• 负责人: An-Shan Hsiao
• 金额: $ 53.54万
• 依托单位: University of Birmingham
• 依托单位国家: 英国
• 项目类别: Fellowship
• 财政年份: 2024
• 资助国家: 英国
• 起止时间: 2024 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FZ514986%2F1
• 关键词: Protein; disorder; crop; stress; adaptation

Global climate change has caused severe weather events such as droughts and high temperatures. These environmental stresses have severe impact on crop plant growth and food security such as fewer grains and smaller crop yields. Investigating how crop plants respond to environmental challenges is a fundamental issue in plant biology research and will raise strategic thinking for future sustainable agriculture. This proposal aims at elucidating the role of protein disorder in crop plant stress responses as well as the mechanisms of action of the macromolecules involved in the underlying biological processes.Intrinsically disordered proteins (IDPs) are a group of proteins natively lacking defined three-dimensional structures. The disordered features enable IDPs to have conformational flexibility and quick responsiveness to environmental stresses, thus serve as regulatory hubs and interact with various partners depending on different circumstances. Literatures suggest that IDPs may play critical roles in plant adaptation to environmental challenges. For example, the abiotic stress-tolerant bioenergy crop switchgrass and the desiccation-tolerant resurrection grass have the highest proportion of proteins with intense disorder. Furthermore, tardigrade disordered proteins have been shown to play a crucial role in surviving desiccation. Disordered dehydrins have also been shown to protect plants under dehydration stress conditions. The precise functions and mechanisms of action are still largely unknown.To understand the mechanism of IDPs involved in plant stress responses, the structural tool nuclear magnetic resonance (NMR) spectroscopy will be used in this study to map the conformational dynamics of IDPs and describe the mechanisms. Three stress responsive IDPs from rice and barley will be used as case studies to provide structural insight into protein disorder in plant stress responses. Within this objective, a breakthrough technology in plant cell NMR method will be developed, which will benefit general plant biologists in terms of monitoring of plant protein dynamics and interaction in vivo in both the time and space dimensions and investigation on molecular mechanism of various developmental processes and stress responses.IDPs are key triggers of liquid-liquid phase separation (LLPS) complexes, also known as biomolecular condensates, which allow the spatiotemporal organization of biochemical reactions by concentrating macromolecules locally. In this proposed research, proximity labeling of two stress-induced IDPs followed by affinity purification and identification of the protein components of LLPS complexes and verification of their interactions and LLPS properties via NMR and cell biology tools will address the function of LLPS in plant stress responses.Since the current IDP databases are mainly focused on mammalian cells and biomedically related proteins, this study will establish the database regarding crop stress responsive IDPs, starting from rice and extending to other crops, which will benefit plant biologists who work on crop science and stress biology.Given the increasing periods of heat and drought due to global climate change, it has become important to understand the strategies that crop plants utilize to cope with various stresses. The proposed research will provide new methods to study plant IDPs in stress responses, fundamental knowledge and mechanistic insights into plant stress physiology, and novel ideas for facing global climate change and solving food security problems.

全球气候变化导致了恶劣的天气事件，例如干旱和高温。这些环境压力对农作物植物的生长和粮食安全产生了严重影响，例如谷物较少和农作物产量较小。研究农作物如何应对环境挑战是植物生物学研究中的一个基本问题，并将为未来的可持续农业提高战略思维。该建议旨在阐明蛋白质疾病在作物植物胁迫反应中的作用以及与潜在的生物学过程有关的大分子的作用机制。跨跨界无序的蛋白质（IDP）是一组原始缺乏的蛋白质，这些蛋白质缺乏定义的三维结构。无序的特征使IDP能够具有构象的灵活性和对环境压力的快速反应性，因此可以作为调节中心，并根据不同情况与各种伴侣互动。文献表明，国内流离失所者可能在适应环境挑战的植物适应中起关键作用。例如，非生物耐应力的生物能源式草原和耐水化的复活草的蛋白质比例最高。此外，已证明Tardigrade无序蛋白在生存的干燥中起着至关重要的作用。还显示脱氢蛋白无序可在脱水应激条件下保护植物。精确的作用和作用机制仍然是未知的。要了解参与植物应力反应的IDP的机制，本研究将使用结构工具核磁共振（NMR）光谱仪来映射IDPS的构象动力学并描述机制。大米和大麦的三个压力反应迅速的IDP将用作案例研究，以提供对植物压力反应中蛋白质疾病的结构洞察力。 Within this objective, a breakthrough technology in plant cell NMR method will be developed, which will benefit general plant biologists in terms of monitoring of plant protein dynamics and interaction in vivo in both the time and space dimensions and investigation on molecular mechanism of various developmental processes and stress responses.IDPs are key triggers of liquid-liquid phase separation (LLPS) complexes, also known as biomolecular condensates, which allow the生化反应的时空组织通过局部浓缩大分子。在这项拟议的研究中，在LLPS复合物的蛋白质成分以及通过NMR和细胞生物学工具对其相互作用和LLP的相互作用和LLP的验证的近端标记，然后识别和鉴定其蛋白质成分，并将LLP在植物压力响应中的功能解决当前的IDP数据群体，并介绍了MAMMAL的群体，并将其介绍为基础。从大米到延伸到其他农作物的作物压力反应迅速的IDP，这将使从事作物科学和压力生物学工作的植物生物学家有益。启动由于全球气候变化而增加的热量和干旱时期，了解作物植物利用来应对各种压力的策略变得很重要。拟议的研究将提供新的方法来研究植物IDP，以应对压力反应，基本知识和对植物压力生理学的机理见解，以及面对全球气候变化和解决粮食安全问题的新思想。