3D STRUCTURE OF CHROMATIN

染色质的 3D 结构

• 批准号: 3302840
• 负责人: CHRISTOPHER L. WOODCOCK
• 金额: $ 13.42万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 1990
• 资助国家: 美国
• 起止时间: 1990-04-01 至 1993-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3302840
• 关键词: DNA; DNA binding protein; Urodela; X ray crystallography; cell growth regulation; cell nucleus; conformation; electron microscopy; eukaryote; genetic regulation; image processing; nucleic acid sequence; nucleosomes; stainings; tomography

This proposal concerns the three dimensional arrangement and detailed structural organization of components within the cell nucleus. The nucleus is a complex organelle that contains, in the form of specific DNA sequences, the information needed for cell construction, maintenance, and replication. Although recent advances in molecular biology have revealed many of the details of gene structure and the decoding and transmission of genetic information, knowledge of the spatial framework within which these mechanisms operate has lagged behind. This is impeding a full understanding of the process of gene activation and control. Experiments are proposed to determine the three dimensional structures and spatial interrelationships within the nucleus. Electron microscopy will be combined with tomography and computer image processing to generate 3D reconstructions of nuclear components, with particular emphasis on the DNA- protein complex known as chromatin. Preparative methods shown to perturb the native structures as little as possible will be employed, and the work will be carried out using sources of nuclei that will be employed, and the work will be carried out using sources of nuclei that are most likely to reveal the basic structural principles of chromatin organization.

该建议涉及三维安排和详细的安排 细胞核内组件的结构组织。 核 是一种复杂的细胞器，包含特定DNA的形式 序列，细胞构造所需的信息，维护和 复制。 尽管分子生物学的最新进展揭示了 基因结构的许多细节以及解码和传播 遗传信息，对这些空间框架的了解 机制运行已落后。 这阻碍了 了解基因激活和控制过程。 提出了实验以确定三维结构和 核内的空间相互关系。 电子显微镜将是 结合层析成像和计算机图像处理，生成3D 核成分的重建，特别强调DNA- 蛋白质复合物称为染色质。 扰动的准备方法 将尽可能少的土著结构和工作 将使用将要使用的核的来源进行，然后 将使用最有可能的核来源进行工作 揭示染色质组织的基本结构原理。