MULTINUCLEAR NMR STUDIES OF CATION-DNA INTERACTIONS

阳离子-DNA 相互作用的多核 NMR 研究

• 批准号: 3297961
• 负责人: WILLIAM H BRAUNLIN
• 金额: $ 14.85万
• 依托单位: POLYTECHNIC UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-09-20 至 1990-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3297961
• 关键词: DNA; cations; chemical chain length; cobalt; conformation; nuclear magnetic resonance spectroscopy; nucleic acid sequence; nucleic acid structure; nucleobase; polyamines; synthetic nucleic acid

The primary objectives of this project are to characterize multivalent cation binding environments on DNA and to explore how these environments change over the course of a biologically important DNA structural transition, the B-Z transition. Specifically, a series of 59 Co NMR measurements are proposed in order (l) to clarify the 59 Co NMR relaxation behavior of Co (NH3)63+ bound to DNA, in particular with respect to the influence of cation mobility on the surface of the DNA (2) to examine base composition, sequence and DNA length effects on Co(NH3)63+ binding strength and heterogeneity and (3) to monitor the state of Co(NH3)63+ over the B-Z transition of synthetic DNA. Additional experiments are proposed using 13C and 15N NMR measurements in order (1) to characterize the dilute-solution behavior of specifically labeled polyamines (2) to determine the local pKa's of polyamines bound to DNA (3) to characterize polyamine binding to natural, synthetic and oligomeric DNA's (4) to examine the mobility of polyamines trapped near the DNA surface and (5) to probe polyamine involvement in the B-Z transition.

该项目的主要目标是表征 DNA 上的多价阳离子结合环境并探索如何 这些环境在生物过程中发生变化 重要的DNA结构转变，B-Z转变。 具体而言，在 中提出了一系列 59 Co NMR 测量 命令(l)阐明Co的59 Co NMR弛豫行为 (NH3)63+ 与 DNA 结合，特别是在影响方面 DNA 表面的阳离子迁移率 (2) 以检查碱基 组成、序列和 DNA 长度对 Co(NH3)63+ 结合的影响 强度和异质性以及（3）监控状态 Co(NH3)63+ 完成合成 DNA 的 B-Z 转变。额外的 实验建议使用 13C 和 15N NMR 测量 命令 (1) 来表征稀溶液行为 特别标记的多胺 (2) 以确定局部 pKa 与 DNA 结合的多胺 (3) 来表征多胺结合 天然、合成和寡聚 DNA (4) 来检查 DNA 表面附近多胺的迁移率和 (5) 探针多胺参与 B-Z 转变。