MECHANISM OF DC AND PULSED IONTOPHORESIS

直流电和脉冲离子电渗疗法的机制

• 批准号: 3297908
• 负责人: RONALD R BURNETTE
• 金额: $ 11.16万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-07-01 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3297908
• 关键词: athymic mouse; drug delivery systems; drug screening /evaluation; human tissue; ion transport; iontophoresis therapy; membrane potentials; skin; thyrotropin releasing hormone

The long range objective of this research is to understand, on a mechanistic level, how drugs can be delivered through the skin while achieving therapeutic systemic blood levels in a controlled and enhanced manner. The method and manner by which transport enhancement will be investigated is by the application of a safe low voltage (iontophoresis) across excised human and nude mouse skin. The nude mouse skin will be evaluated as an alternative preclinical method for screening drug transport by iontophoresis. Both DC (constant current) and pulsed current modes of iontophoretic drug delivery will be investigated and compared. In particular, the skin's ion transport selectivity, the ion's transport pathway through the skin, the effects of enhanced charge transport on the skin, and the effects of the co- administration of iontophoresis and a model skin permeability enhancer on the skin will be determined. This information will allow the drug transport efficiencies and tissue alteration effects of constant current and pulsed iontophoresis to be assessed. An optimal iontophoretic delivery protocol will then be developed which maximizes drug delivery and minimizes tissue alteration. This protocol will be tested by using a model peptide drug and determining its iontophoretic transport across human skin.

这项研究的远程目标是了解 机械水平，如何通过皮肤输送药物 同时在受控的 和增强的方式。 方法和方式 运输增强将通过应用程序进行调查 在切除的人类和 裸鼠皮肤。 裸鼠皮肤将被评估为 通过筛查药物运输的替代临床前方法 离子噬菌体。 直流（恒定电流）和脉冲电流 将研究离子噬药物输送模式，并 比较的。 特别是皮肤的离子传输选择性， 离子的运输路径穿过皮肤，增强的影响 对皮肤的电荷运输以及共同的影响 离子噬菌体和模型皮肤渗透性的给药 将确定皮肤上的增强剂。 这些信息将 允许药物转运效率和组织改变效应 待评估的恒定电流和脉冲离子电池。 一个 然后将开发最佳的离子噬菌输送方案 这可以最大化药物输送并最大程度地减少组织的改变。 该方案将通过使用模型肽药物和 确定其跨人类皮肤的离子遗迹转运。