BIOPHYSICAL ASPECTS OF ELECTRIC FIELD BIOEFFECTS

电场生物效应的生物物理方面

基本信息

批准号：
3292671
负责人：
Morton Miller
金额：
$ 6.98万
依托单位：
UNIVERSITY OF ROCHESTER
依托单位国家：
美国
项目类别：
财政年份：
1987
资助国家：
美国
起止时间：
1987-07-01 至 1990-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/3292671
关键词：
adenosinetriphosphatase biological models cell membrane electric field electromagnetic radiation erythrocytes growth /development ion transport membrane permeability membrane potentials radiobiology root sodium potassium exchanging ATPase

项目摘要

Adverse biological effects resulting from exposure to extremely low frequently (ELF) electric fields (EF) have been claimed. There is strong evidence that the EF-induced membrane potential is involved in induction of these effects. Plant roots provide a model cell system in which ELF-EF effects are observed; growth inhibition is correlated with EF-induced membrane potential magnitude. Study of roots from two species indicates a threshold at EF-induced membrane potential 3 mV; at EF-induced membrane potential 10 mV, growth nearly ceases. The project will explore a possible mechanism by which EF- induced membrane potential are transduced into growth effects. Mediated H+ efflux from plant cells is involved in cellular growth. The effect of ELF-EF exposure on H+ efflux will be studied, and will provide a basis for comparison of EF effects on ion transport in roots and erythrocytes, in which EF-induced membrane potential affect Na/K transport. The project will also quantify the relationship between EF-induced membrane potential and growth effect magnitude in roots of several genera, assess interspecific differences, and test a general model of the relationship. Exposure conditions likely to induce comparable EF- induced membrane potential in other systems will be calculable. The effect of ELF-EF exposure on H+ efflux from roots will be examined by measuring the rate at which EF-exposed and sham- exposed root tip suspensions acidify a weakly-buffered suspension fluid; it is hypothesized that EF exposures which inhibit root growth will inhibit or reverse H+ efflux from the roots. A dose- response relationship will be sought for any such effect observed, and the results related to the mean EF-induced membrane potential in the root tips. The relationship between EF-induced membrane potential and growth effect magnitude will be examined in roots of 4 species by measuring the growth rates of marked segments within the elongation zone following ELF-EF exposure/sham-exposure onset at various EF strengths. Segmental growth rates will be related to the mean EF-induced membrane potential in the segment, the latter calculated on the basis of the applied EF strength and the mean size of cells in the segment. Cell size data will be obtained from serial histological sections.

暴露于极端的生物学影响人们宣称经常低（EF）的电场（EF）。有充分的证据表明EF诱导的膜电位参与了这些影响的诱导。植物根提供了一种模型细胞系统，在其中效果观察到；生长抑制与EF诱导的膜电位大小。研究两个物种的根表示在EF诱导的膜电位3 mV处的阈值；在 EF诱导的膜电位10 mV，生长几乎停止。该项目将探讨一种可能的机制诱导的膜电位被引发到生长效应中。植物细胞中介导的H+外排参与细胞生长。将研究ELF-EF暴露对H+外排的影响，并将将提供比较EF对离子传输影响的基础在根和红细胞中，其中EF诱导的膜潜在影响Na/k运输。该项目还将量化 EF引起的膜电位与生长效应幅度在几个属的根中，评估种间差异，并测试关系。暴露条件可能引起可比的EF- 在其他系统中诱导的膜电位将是可以计算的。 ELF-EF暴露对根部H+外排的影响将是通过测量EF暴露和假的速率来检查暴露的根尖悬浮液会酸酸悬浮液体液;假设EF暴露会抑制根部生长会抑制或反向根部的H+外排。剂量 - 观察到的任何此类效果将寻求响应关系，以及与平均EF诱导的膜有关的结果根尖的潜力。 EF引起的关系膜电位和生长效应幅度将是通过测量 ELF-FEF之后的伸长区域内的明显段在各种EF强度下的暴露/假曝光发作。分段增长率将与平均EF诱导的片段中的膜电位，后者在施加的EF强度和平均细胞中的基础部分。细胞尺寸数据将从串行组织学获得部分。