BIOPHYSICAL BASES OF PULSED ULTRASOUND BIOEFFECTS

脉冲超声生物效应的生物物理基础

• 批准号: 3178018
• 负责人: Morton Miller
• 金额: $ 33.52万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3178018
• 关键词: 6 thioguanine; Anura; Cyanophyta; X ray; biological models; biophysics; chick embryo; chromosome aberrations; cytogenetics; diagnosis design /evaluation; diagnosis procedure safety; embryo /fetus cell /tissue; heart disorder; kidney disorder; laboratory mouse; laboratory rat; myelinopathy; node of Ranvier; sound impedance; ultrasonography; ultrasound biological effect

This application's long term objective is to determine the potential for diagnostic ultrasound to induce biological effects in mammalian tissues. To accomplish this objective, several approaches are proposed; 1) to determine for extra utero human placentas the size and quantily of gas nuclei and the ultrasound parameters (IM, frequency, prr) needed to activate the nuclei to acoustic cavities; 2) to determine (a) the role of intercellular space and gas in mediating acoustic cavitation in an in vitro multicellular tissue system (multicell spheroids), to measure the force requisite for bubble penetration of cells, (c) to determine whether acoustic cavitation occurs intracellularly; 3) to verify certain phenomenological reports regarding ultrasound-induced effects (sister chromatid exchanges, unscheduled DNA systhesis, cell transformation, multigeneration cell deformation) in certain in vitro systems and, if verified, to identify the physical mechanism of action causing the effects(s); 4) to investigate the postulate that at hyperthermic temperatures a non-cavitational, non-thermal ultrasound-induced mechanism is operative; and 5) to determine the most effective pulsing regimes (prr, frequency) for induction cavitation at ultrasound intensities (Im) comparable to those emitted by clinical diagnostic units. The results obtained should be directly relevant to assessments of the potential for diagnostic ultrasound to induce biological effects in human tissue. Because the proposed projects focus on understanding how certain effects in chemical, in vitro, and vivo mammalian systems are produced (i.e., mechanisms) the results from these areas may be extrapolated to broader question of health and safety from exposure to dignostic ultrasound.

该应用程序的长期目标是确定 诊断超声诱导哺乳动物组织的生物学作用。到 实现这一目标，提出了几种方法； 1）确定 对于额外的子宫，人胎盘的大小和数量的气核和数量 超声参数（IM，频率，PRR）需要激活核至 声腔； 2）确定（a）细胞间空间和 体外多细胞组织中介导的气水中的气体 系统（多细胞球体），以测量气泡所需的力 细胞的穿透，（c）确定是否发生声气水 细胞内； 3）验证有关某些现象学报告 超声诱导的效果（姐妹染色单体交换，外面的DNA 在某些 体外系统，如果经过验证，以确定 行动导致效果； 4）调查假设 高温温度非浪费，非热超声诱导的 机制是手术的； 5）确定最有效的脉冲 超声强度下的感应空化的制度（PRR，频率） （IM）与临床诊断单元发射的那些相当。 获得的结果应与评估直接相关 诊断超声引起人类生物学作用的潜力 组织。 因为拟议的项目专注于了解如何确定 产生化学，体外和体内哺乳动物系统的影响 （即机制）这些区域的结果可能被外推到 从暴露于Dignostic Ultrasound的健康和安全性问题。