IMMUNOGLOBULIN GENES IN DIFFERENTIATING B CELLS

分化 B 细胞中的免疫球蛋白基因

• 批准号: 3290664
• 负责人: NAOMI ROSENBERG
• 金额: $ 15.76万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1991-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3290664
• 关键词: Abelson leukemia virus; B lymphocyte; cell differentiation; flow cytometry; genetic manipulation; humoral immunity; immunoglobulin G; immunoglobulin M; immunoglobulin genes; leukocyte activation /transformation; tissue /cell culture

A series of important changes in the structure of immunoglobulin (Ig) genes occurs during B cell development. Both formation of complete variable (V) regions 5' of the constant (C) regions encoding both heavy (H) and light chain proteins and the subsequent linkage of the V-H region with C-H regions 3' of C (heavy chain switching) occur by recombinatorial mechanisms. However, because these events are temporally distinct and use different recognition sequences, different recombinatorial systems are probably used in each case. To date, most of our information concerning the mechansim of H chain switching and its timing during the course of B cell differentiation has come from studies of myelomas and a few B cell tumors. We have recently developed a model system to study Ig gene structure during differentiation of mature B cells using the retrovirus Abelson virus. Using this approach, we have developed a series of clonal, immortalized, nontumorigenic mIgM and IgG-positive B cell lines. Different isolates synthesize various H and L chain isotypes and some isolates secrete Ig. The unique nature of these cells and their ability to carry out a key recombinatorial step in B cell development make them an ideal model system to study both the timing and the mechanism of deletional switching during B cell differentiation.

免疫球蛋白（Ig）基因结构的一系列重要变化 发生在 B 细胞发育过程中。 两者形成完全变量 (V) 编码重链 (H) 和轻链的恒定 (C) 区的 5' 区 链蛋白以及随后 V-H 区与 C-H 的连接 C 的 3' 区（重链转换）通过重组发生 机制。 然而，由于这些事件在时间上是不同的并且使用 不同的识别序列，不同的重组系统 可能在每种情况下都使用。 迄今为止，我们的大部分信息都涉及 B过程中H链切换的机制及其时机 细胞分化来自对骨髓瘤和一些 B 细胞的研究 肿瘤。 我们最近开发了一个模型系统来研究 Ig 基因结构 使用逆转录病毒 Abelson 病毒分化成熟 B 细胞。 使用这种方法，我们开发了一系列克隆、永生化、 非致瘤性 mIgM 和 IgG 阳性 B 细胞系。 不同的分离株 合成各种 H 和 L 链同种型，一些分离株分泌 Ig。 这些细胞的独特性质及其执行关键任务的能力 B 细胞发育中的重组步骤使其成为理想的模型系统 研究 B 期间删除转换的时间和机制 细胞分化。