BACTERIAL CELL MEMBRANE: GROWTH AND CELL DIVISION

细菌细胞膜：生长和细胞分裂

• 批准号: 3284661
• 负责人: MOSELIO SCHAECHTER
• 金额: $ 16.76万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-07-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3284661
• 关键词: Escherichia coli; Salmonella typhimurium; bacterial DNA; bacterial genetics; bacteriophage lambda; cell cycle; cell growth regulation; cell membrane; density gradient ultracentrifugation; electron microscopy; gel electrophoresis; membrane structure; microorganism growth; mutant; plasmids; radiotracer

We have recently succeeded in isolating the replicative origin of the Escherichia coli chromosome. The complex is composed of the DNA at and near the origin, and various proteins. We call this complex OC, for "origin complex". We will attempt further characterization of the OC particles. specifically, we will purify them with regard to protein content, and attempt to characterize the intrinsic proteins of these particles. Preliminary results have shown that partially purified DNA from OC binds to outer membrane, while other DNA does not. We will use a filter-binding assay to determine further the specificity and other properties of this interaction.

我们最近成功地隔离了 大肠杆菌染色体。 该络合物由DNA在 起源和各种蛋白质。 我们称此复杂的OC为“原点复杂”。 我们将尝试进一步表征OC颗粒。 具体来说，我们 将在蛋白质含量方面净化它们，并试图表征 这些颗粒的内在蛋白质。 初步结果表明 OC的部分纯化DNA与外膜结合，而其他DNA则DNA DNA 不是。 我们将使用过滤器结合测定法来进一步确定特异性 和这种相互作用的其他属性。