Quantifying how host genotype and microbiome composition combine to influence susceptibility to Dothistroma needle blight disease in pine trees.

量化宿主基因型和微生物组组成如何结合影响松树对针叶枯病的易感性。

• 批准号: BB/W020610/1
• 负责人: Sue Jones
• 金额: $ 55.05万
• 依托单位: James Hutton Institute
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FW020610%2F1
• 关键词: Quantifying; how; host; genotype; microbiome

A key challenge in plant disease research is to understand how the interactions between the plant host and its associated microbiome (the bacteria, fungi and viruses that exist within/on the plant) affect plant disease incidence and severity. These interactions are affected by genetic variation in the host, environmental conditions, and the variety microbes in the host's microbiome. For this project we focus on the pine tree disease Dothistroma needle blight (DNB), that detrimentally affects the growth and life span of pine species worldwide. As trees will also play a critical role in combatting our current biodiversity and climate crises, an understanding of the factors governing the arrival and spread of tree diseases, and how they impact tree health, are important policy strategies. Innovative research on tree health, as proposed in our project, will deliver to these strategies.DNB is caused by Dothistroma septosporum, a UK listed quarantine fungus that is now found worldwide, where it affects more than 100 tree species including the commercial timber species Scots pine, Corsican pine and Lodgepole pine. DNB is a foliar disease affecting the pine needles that causes a variety of symptoms including premature needle loss. These symptoms result in reduced timber yields and can cause tree death. Currently the only treatment for DNB is tree stand management to reduce the favourable conditions for spread and the use of fungicides on nursery trees. In this project we will test our hypothesis that the genetics of the host tree drives microbiome composition and interactions of the pine needles to alter the trees susceptibility to DNB. To achieve this we will combine existing host genetic data from our established Scots pine tree trial with new nucleic acid sequencing information on the pine needle microbiome. We will use meta-genomic and meta-transcriptomic sequencing. Meta-genomic sequencing provides a microbe inventory ("what is there?") and meta-transcriptomic sequencing tell us which community members are active ("what are they doing?"). We have three specific objectives 1) quantify the extent to which host genetic variation explains variation in microbial community composition and susceptibility to DNB, (2) evaluate temporal variation in microbiome composition and function during D. septosporum infection and (3) predict which microbiome community members interact with D. septosporum and which impact on host susceptibility to DNB.Our proposal addresses key challenges within the BBSRC integrative microbiome research priority (1) relating host genotype to microbiome composition and health status, and (2) characterising functional properties of the microbiome and the genes driving the microbiome-host relationship. The benefits of this proposal are two-fold: (a) we will understand how host genetics and microbiome composition combine to influence susceptibility to DNB disease in pine trees and (b) we will develop publicly available methods to integrate multi-omics (metagenomics, meta-transcriptomics and genotyping) data which will be directly applicable to many different host-microbiome systems, from plants to humans. The project will benefit forest researchers with interests in tree disease management and agricultural researchers with interests in the effect of crop genetics on microbiomes and crop disease incidence and severity. The project will also benefit computational biologists and biologists integrating metagenomics, transcriptomics and genotyping datasets to answers diverse biological questions.

植物疾病研究中的一个主要挑战是了解植物宿主与其相关微生物组（植物内部/植物内存在的细菌，真菌和病毒）之间的相互作用如何影响植物性疾病的发生率和严重程度。这些相互作用受宿主，环境条件和宿主微生物组中多种微生物的遗传变异的影响。对于这个项目，我们关注的是松树疾病Dothisroma deadle Blight（DNB），对全球松树物种的生长和生命跨度有不利影响。由于树木还将在打击我们当前的生物多样性和气候危机，了解有关树木疾病到来和传播的因素以及如何影响树木健康的因素方面发挥关键作用，这是重要的政策策略。正如我们项目中提出的那样，对树木健康的创新研究将传递到这些策略。dnb是由英国列出的隔离真菌Dothistroma Septosporum引起的，现在在全球范围内发现，它影响了100多种树种，包括商业木材物种Scots Scots Pine，Corsican Pine和Lodgepole Pine。 DNB是一种影响松针的叶面疾病，会导致多种症状，包括过早的针头丧失。这些症状导致木材产量降低，并可能导致树木死亡。目前，DNB唯一的治疗方法是树立林木管理公司，以减少育树树上传播和使用杀菌剂的有利条件。在这个项目中，我们将检验我们的假设，即宿主树的遗传学驱动微生物组的组成以及松针的相互作用，以改变树木对DNB的敏感性。为了实现这一目标，我们将结合我们已建立的苏格兰松树试验中现有的宿主遗传数据，并将新的核酸测序信息与松针微生物组有关。我们将使用元基因组和元转录组测序。元基因组测序提供了微生物库存（“什么？”）和元文字组测序告诉我们哪些社区成员是活跃的（“他们在做什么？”）。我们有三个特定的目标1）量化宿主遗传变异的程度解释了微生物群落组成和对DNB的敏感性的变化，（2）评估d。spososporum感染过程中微生物组组成和功能的时间变化，以及（3）预测哪些微生物组成员与D. sptosporum的相互作用以及对宿主的挑战的相互作用，以及对宿主的挑战的相互作用。优先级（1）将宿主基因型与微生物组组成和健康状况有关，以及（2）表征微生物组的功能特性和驱动微生物组宿主关系的基因。该提案的好处是两倍：（a）我们将了解宿主遗传学和微生物组组成如何结合使用松树中的DNB疾病的易感性，（b）我们将开发公开可用的方法来将多摩学（元元素学，元文字和基因型）整合到许多不同的宿主系统中，这些方法将直接适用于许多不同的宿主系统。该项目将使对树木疾病管理有兴趣的森林研究人员和对农作物遗传学对微生物组和作物疾病发病率和严重程度的影响感兴趣的农业研究人员有利。该项目还将受益于计算生物学家和生物学家整合宏基因组学，转录组学和基因分型数据集以回答各种生物学问题的数据集。