Harnessing Yarrowia metabolism to generate high value terpenoids

利用耶氏酵母代谢产生高价值萜类化合物

• 批准号: BB/T013176/1
• 负责人: Rodrigo Ledesma Amaro
• 金额: $ 67.01万
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FT013176%2F1
• 关键词: Harnessing; Yarrowia; metabolism; generate; value

Lutein, a pigmented carotenoid with antioxidant effects, is extensively used in food and animal feed. Naturally, it is produced in different plants and algae. Animals, including humans, do not synthetase its own lutein, however, this compound is essential for health. At the moment lutein for food & feed or pharmaceutical usage is extracted from marigold flowers (Tagetes spp.), where it is accumulated in low amounts and which requires a vast amount of land and fertilisers, making it a very expensive product. So far, an efficient biotechnological production process does not exist. The aim of the project is to develop, for the first time, the production of lutein using engineered microorganisms. Using state of the art synthetic biology methods for genome engineering we will transfer the capacity to synthesise lutein from plants to the in industrial yeast Yarrowia lipolytica. The production will be further optimised by screening different combination of plant genes in order to find the right balance of them that leads to high amounts of this valuable compound. The strains harbouring the best combinations, therefore the best producers, will be studied in industrial-like conditions using bioreactors, where the production can be optimised and maximised. In addition, we will explore the capacity to produce lutein using waste streams from other industries (i. e. Glycerol from biodiesel production), which will help to develop a circular process and to reduce generated waste.The project will be done in close collaboration between the group of Dr Rodrigo Ledesma-Amaro from Imperial College London and Acies Bio, one of the leading industrial biotech companies specialised in the development of new strains and bioprocesses.

叶黄素是一种具有抗氧化作用的有色类胡萝卜素，广泛用于食品和动物饲料中。当然，它是在不同的植物和藻类中产生的。包括人类在内的动物本身不会合成叶黄素，但这种化合物对健康至关重要。目前，用于食品和饲料或医药用途的叶黄素是从万寿菊花（Tagetes spp.）中提取的，其积累量很少，并且需要大量的土地和肥料，使其成为一种非常昂贵的产品。迄今为止，还不存在有效的生物技术生产工艺。该项目的目的是首次开发利用工程微生物生产叶黄素。使用最先进的基因组工程合成生物学方法，我们将把从植物合成叶黄素的能力转移到工业酵母解脂耶氏酵母中。将通过筛选植物基因的不同组合来进一步优化生产，以找到它们的适当平衡，从而产生大量这种有价值的化合物。具有最佳组合的菌株，即最佳生产者，将在类似工业条件下使用生物反应器进行研究，其中生产可以得到优化和最大化。此外，我们将探索利用其他行业的废物流（即生物柴油生产中的甘油）生产叶黄素的能力，这将有助于开发循环工艺并减少产生的废物。该项目将在集团之间密切合​​作下完成伦敦帝国理工学院的 Rodrigo Ledesma-Amaro 博士和 Acies Bio 公司是专门从事新菌株和生物工艺开发的领先工业生物技术公司之一。

项目成果

Additional file 1 of Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation

用于复杂基质降解的微生物群落中的资源感知全细胞分工模型的附加文件 1

• DOI: http://dx.10.6084/m9.figshare.20064670
• 发表时间: 2022
• 作者: Atkinson E

Resource-aware whole-cell model of division of labour in a microbial consortium for complex-substrate degradation.

用于复杂基质降解的微生物群落中的资源感知全细胞分工模型。

• DOI: http://dx.10.1186/s12934-022-01842-0
• 发表时间: 2022
• 期刊: Microbial cell factories
• 影响因子: 6.4
• 作者: Atkinson E

Synergies of Systems Biology and Synthetic Biology in Human Microbiome Studies.

系统生物学和合成生物学在人类微生物组研究中的协同作用。

• DOI: http://dx.10.3389/fmicb.2021.681982
• 发表时间: 2021
• 期刊: Frontiers in microbiology
• 作者: Ezzamouri B

Production of human milk fat substitute by engineered strains of Yarrowia lipolytica.

利用解脂耶氏酵母工程菌株生产人乳脂肪替代品。

• DOI: http://dx.10.1016/j.mec.2022.e00192
• 发表时间: 2022
• 期刊: Metabolic engineering communications
• 影响因子: 5.2
• 作者: Bhutada G

Quorum sensing in synthetic biology: A review

合成生物学中的群体感应：综述

• DOI: http://dx.10.1016/j.coisb.2021.100378
• 发表时间: 2021
• 期刊: Current Opinion in Systems Biology
• 影响因子: 3.7
• 作者: Boo A