ELECTROCHEMISTRY OF PURINES

嘌呤的电化学

• 批准号: 3270220
• 负责人: GLENN DRYHURST
• 金额: $ 11.04万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3270220
• 关键词: adenine; chemical substitution; deoxyribose; electrical measurement; electrical potential; electrochemistry; gas chromatography; guanine; high performance liquid chromatography; hypoxanthines; mass spectrometry; oligonucleotides; oxidation; peroxidases; purine /pyrimidine metabolism; purine nucleosides; purine nucleotides; ribose; ribose phosphate; urate; xanthines

It is proposed to continue investigations into the electrochemical and in some instances the enzymatic oxidation of a number of biologically significant purine bases, nucleosides, nucleotides and oligonucleotides. The electrochemical methodology to be employed will include linear and cyclic sweep voltammetry, large scale and thin-layer controlled potential electrolysis and coulometry, thin-layer spectroelectrochemistry, various potentiostatic techniques and other appropriate methods. The electrochemical techniques will be complemented by a range of spectral, kinetic and other analytical methods to allow the characterization and identification of intermediates and products formed as a result of the electrooxidation reactions. Of particular importance in characterizing and identifying intermediates will be a technique based on a combination of thin-layer spectroelectrochemistry to generate UV-absorbing intermediates. The intermediate(s) will then be trapped by rapid freezing of the solution, lyophilization and then analyzed by forming appropriate silylated derivatives followed by separation and analysis by gas chromatography-mass spectrometry. Products will be analyzed using a broad range of liquid and gas chromatography methods, gas chromatography-mass spectrometry and various other spectral techniques. It is anticipated that the spectral, kinetic and analytical techniques developed to follow the course of the electrochemical oxidation reactions will also be readily adapted to monitoring the course of enzyme-catalyzed oxidations of purines. The long range goal of this research is to develop a clear and fundamental understanding of the oxidation chemistry of naturally occurring purine derivatives using electrochemical techniques and to demonstrate, by the investigation of various enzyme-catalyzed oxidations, that the oxidation chemistry developed on the basis of electro-chemical studies can provide detailed and uniquely valuable insights into the biological reactions of these compounds.

建议继续调查电化学和 某些实例将许多生物学上的酶促氧化 明显的嘌呤碱基，核苷，核苷酸和寡核苷酸。 要使用的电化学方法将包括线性和 循环扫描伏安法，大规模和薄层控制电势 电解和库尔仪，薄层光谱化学，各种 电位技术和其他适当的方法。 这 电化学技术将得到一系列光谱的补充， 动力学和其他分析方法，以允许表征和 鉴定由 电氧化反应。 在表征和 识别中间体将是一种基于组合的技术 薄层的光谱电化学可产生紫外线吸收中间体。 然后，中级将被溶液快速冻结所困， 冻干，然后通过形成适当的硅胶分析 衍生物随后分离和通过气相色谱质量分析 光谱法。 产品将使用广泛的液体进行分析， 气相色谱法，气相色谱 - 质谱法和 各种其他光谱技术。 预计光谱， 动力学和分析技术旨在遵循 电化学氧化反应也将很容易适应 监测嘌呤的酶催化氧化的过程。 长 这项研究的范围目标是开发一个清晰而基本的 了解天然嘌呤的氧化化学 使用电化学技术的衍生物，并通过 研究各种酶催化的氧化的研究，即氧化 根据电化学研究开发的化学反应可以提供 详细且独特的有价值的见解对生物学反应 这些化合物。