IMMUNOGENETIC STUDIES OF THE HUMAN MHC

人类 MHC 的免疫遗传学研究

• 批准号: 3268099
• 负责人: Frances E Ward
• 金额: $ 26.74万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1974
• 资助国家: 美国
• 起止时间: 1974-10-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268099
• 关键词: African American; B lymphocyte; T lymphocyte; alleles; autosomal recessive trait; biological polymorphism; gene interaction; genetic markers; graft versus host disease; hereditary hemochromatosis; human population genetics; human subject; immunogenetics; laboratory mouse; laboratory rat; leukocyte antigen typing; major histocompatibility complex; monoclonal antibody; neoplasm /cancer genetics; nomenclature; serology /serodiagnosis

T cell activation requires that the immunogen be presented in association with a major histocompatibility complex (MHC) class II molecule. Our long-term objective is to understand how antigen presentation in the context of self applies to alloimmune (transplantation) responses and, given the rich array to human class II molecules, which ones are used for which functions. We will use serologic, cellular, and molecular in approaches to pursue our objective. We will define epitopes of MHC molecules in structural terms using haplotypes with unusual linkage disequilibria and varient alleles that we have identified in different ethnic populations. The epitopes will be defined serologically, using allo- and monoclonal antibodies, and molecularly usng restriction endonucleases and genetic probes, to map and sequence gene segments. Epitopes of MHC molecules will also be investigated for their ability to serve as restriction elements for foreign antigen presentation to T lymphocytes. We will investigate the ability of certain mediators of gene expression to alter differentially the profile of MHC antigen expression and will analyze mechanisms involved in gene regulation of this expression. Lastly, we will investigate genetic basis for disease susceptibility in polyglandular failure disease, hodgkin's disease and type II diabetes using serologic and molecular probes.

T 细胞激活需要免疫原存在于 与主要组织相容性复合体 (MHC) 类别的关联 II 分子。 我们的长期目标是了解抗原如何 自我背景下的表达适用于同种免疫 （移植）反应，并考虑到人类丰富的 II 类分子，哪些分子用于哪些功能。 我们 将使用血清学、细胞和分子方法来追求 我们的目标。 我们将定义 MHC 分子的表位 使用具有不寻常连锁的单倍型的结构术语 我们已经确定的不平衡和变异等位基因 不同种族的人口。 表位将被定义 血清学上，使用同种异体和单克隆抗体，以及 分子使用限制性核酸内切酶和遗传探针， 对基因片段进行图谱和测序。 MHC 分子的表位 还将对其作为限制的能力进行调查 外来抗原呈递至 T 淋巴细胞的元件。 我们 将研究基因某些介质的能力 表达以差异改变 MHC 抗原的分布 表达并分析参与基因的机制 该表达的调节。 最后，我们将研究遗传 多腺体衰竭疾病易感性的基础， 霍奇金病和 II 型糖尿病使用血清学和 分子探针。

项目成果

HLA and malaria in four Colombian ethnic groups.

哥伦比亚四个民族的 HLA 和疟疾。

• DOI: 10.1590/s0036-46651988000500001
• 发表时间: 1988
• 期刊: Revista do Instituto de Medicina Tropical de Sao Paulo
• 影响因子: 1.9
• 作者: Restrepo,M;Rojas,W;Montoya,F;Montoya,AE;Dawson,DV
• 通讯作者: Dawson,DV

Epitopes within the HLA-B5,B35 cross-reacting group.

HLA-B5、B35 交叉反应组内的表位。

• DOI: 10.1016/0198-8859(89)90035-9
• 发表时间: 1989
• 期刊: Human immunology
• 影响因子: 2.7
• 作者: Ward,FE;Stewart,AD;Ruiz,RE;Killian,P;Amos,DB;Johnson,AH;Kostyu,DD
• 通讯作者: Kostyu,DD

Isolation of heavy chain class switch variants of a monoclonal anti-DC1 hybridoma cell line: effective conversion of noncytotoxic IgG1 antibodies to cytotoxic IgG2 antibodies.

单克隆抗 DC1 杂交瘤细胞系的重链类别转换变体的分离：非细胞毒性 IgG1 抗体有效转化为细胞毒性 IgG2 抗体。

• DOI: 10.1016/0198-8859(83)90009-5
• 发表时间: 1983
• 期刊: Human immunology
• 影响因子: 2.7
• 作者: Parham,P;Kipps,TJ;Ward,FE;Herzenberg,LA
• 通讯作者: Herzenberg,LA

Alloantibody responses in multiply transfused sickle cell patients.

多次输血镰状细胞患者的同种抗体反应。

• DOI: 10.1111/j.1399-0039.1987.tb01615.x
• 发表时间: 1987
• 期刊: Tissue antigens
• 作者: Reisner,EG;Kostyu,DD;Phillips,G;Walker,C;Dawson,DV
• 通讯作者: Dawson,DV

Paired H-2-specific monoclonal antibodies react differently to red cells and lymphocytes.

配对的 H-2 特异性单克隆抗体对红细胞和淋巴细胞的反应不同。

• DOI: 10.1007/bf00350121
• 发表时间: 1981
• 期刊: Immunogenetics
• 影响因子: 3.2
• 作者: Tonkonogy,SL;Amos,DB
• 通讯作者: Amos,DB