STRUCTURE AND FUNCTION OF NUCLEIC ACIDS

核酸的结构和功能

• 批准号: 3268152
• 负责人: IGNACIO TINOCO
• 金额: $ 29.43万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-12-01 至 1989-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3268152
• 关键词: chemical structure function; chromatography; circular dichroism; conformation; nuclear magnetic resonance spectroscopy; nucleic acid chemical synthesis; nucleic acid sequence; nucleic acid structure; oligonucleotides; temperature jump; thermodynamics

The goal of this project is to obtain a fundamental understanding of the conformations of nucleic acids and of the forces which govern them. We will seek useful correlations between the base sequence of a nucleic acid and its structure and biological function. A better understanding of nucleic acids may help in the prevention or cure of all diseases which depend on the functions of nucleic acids. These include viral diseases, genetic diseases and cancer. Oligonucleotides of specific base sequence will be synthesized to provide structural elements of DNA, RNA and DNA-RNA hybrids. Thermodynamics and kinetics of formation of complementary double-strand helices, mismatched helices and hairpin loops will be measured. Conformations will be determined in solution using one-and two- dimensional nuclear magnetic resonance measurements. Tertiary and higher-order folded structures in nucleic acids will be studied using absorption and scattering of circularly polarized light.

该项目的目的是获得对 核酸和支配它们的力的构象。 我们 将寻求核酸的碱基序列之间的有用相关性 及其结构和生物学功能。 更好地理解 核酸可能有助于预防或治愈所有疾病 取决于核酸的功能。 这些包括病毒疾病， 遗传疾病和癌症。 特定碱基序列的寡核苷酸将合成以提供 DNA，RNA和DNA-RNA杂种的结构元素。 热力学和 互补双链螺旋形成的动力学，不匹配 将测量螺旋和发夹环。 构象将是 在溶液中使用一和两维核磁磁性确定 共振测量。 核酸中的三级和高阶折叠结构将是 使用吸收和圆形极化光的散射进行了研究。