DETERMINANTS OF OPTIC AXON OUTGROWTH

视轴突生长的决定因素

• 批准号: 3260322
• 负责人: RAYMOND D LUND
• 金额: $ 11.98万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-01 至 1991-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3260322
• 关键词: antibody; axon; cell cell interaction; cell migration; electron microscopy; embryo /fetus; eye transplantation; gene expression; growth inhibitors; histochemistry /cytochemistry; histology; immunochemistry; microscopy; nervous system regeneration; neural plasticity; neuronal transport; tissue /cell culture; visual cortex

An antibody has been identified to a cell surface protein, designated M6, which blocks neurite outgrowth in vitro. Studies on the developing optic nerve in vivo indicate that M6 is expressed only up to a certain critical period of development. The biological function in vitro and the pattern of expression in vivo suggested that by knowing more about the behavior of the antigen and its antibody, we would gain further insight into why optic axons stop growing during development and why they generally fail to regenerate after injury. Additional results collected over the past year confirm this expectation and indicate that a substantial investigation of the antigen and its antibody centered on the primary optic pathway is justified. This proposal focuses on three areas: (1) We wish to examine the hypothesis that the antigen is involved in vivo with the processes of axonal growth and cell migration. (2) We wish to use the antibody to arrest irreversibly both cell migration and axonal outgrowth as a tool for studying various interdependencies occurring during development and after injury. (3) We wish to examine how the context in which a neuron develops may regulate the expression of the M6 antigen and as a result modify neurite outgrowth and cell migration patterns. Nine individual experiments are proposed, each of which addresses one or more of the three areas raised. Collectively, they should provide insight into the behavior in vivo of an antigen which appears to regulate neurite extension by a molecular mechanism which is under investigation in parallel studies. They will also exploit the use of the antibody both as a probe and a label for investigating plasticity and regeneration of the visual system.

已经确定了一种抗体，该抗体被指定为M6的细胞表面蛋白 它可以在体外阻断神经突生长。 开发光学的研究 神经在体内表明M6仅表达至某个临界 发展时期。 体外生物学功能和模式 体内表达表明，通过更多地了解 抗原及其抗体，我们将进一步深入了解为什么光学 轴突在开发过程中停止增长，以及为什么它们通常无法 受伤后再生。 过去一年收集的其他结果 确认这一期望，并表明对 抗原及其抗体以主视途径为中心 有理由。 该提案侧重于三个领域：（1）我们希望研究 假设抗原与体内有关 轴突生长和细胞迁移。 （2）我们希望使用抗体 不可逆转地捕捉细胞迁移和轴突生长作为一种工具 研究在开发过程中和之后发生的各种相互依存关系 受伤。 （3）我们希望研究神经元的背景如何发展 可能调节M6抗原的表达，结果修改 神经突生长和细胞迁移模式。 提出了九个个人实验，每个实验都解决了一个或 在三个区域中，有更多。 他们总体应该提供洞察力 进入似乎调节神经突调节的抗原的体内行为 通过分子机制进行扩展，该机制正在平行研究 研究。 他们还将利用抗体的使用均用作探针 以及用于研究视觉可塑性和再生的标签 系统。