ION & WATER TRANSPORT IN CILIARY EPITHELIUM

离子

• 批准号: 3263734
• 负责人: NASSER A FARAHBAKHSH
• 金额: $ 9.41万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1990-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3263734
• 关键词: acetazolamide; electrophysiology; epithelium; eye pharmacology; intraocular fluid; ion transport; isoproterenol; laboratory rabbit; membrane permeability; microscopy; osmosis; perfusion; secretion; uvea ciliary body

A new method will be used to investigate ion and water transport in the ciliary epithelium cells. A single ciliary process from a pigmented rabbit will be isolated, placed in a specially designed chamber, and rapidly perfused with physiological Ringer. Quantitative light microscopy will be used to measure the changes in the volume of a non-pigmented cell located at the edge of the ciliary process, when the perfusing solution is changed to solutions of different osmolality, composition, or temperature. A combination of volume measurements with the intracellular ion activity measurements will be used to estimate the water permeability of non-pigmented epithelial cells and the rate of transport of the major inorganic ions responsible for volume regulation and aqueous humor formation. The nature of the ion transport mechanisms will be further elucidated by the use of known inhibitors of transport in epithelia. Finally, we will investigate the effects on non-pigmented cells of the pharamcological agents used in the treatment of glaucoma.

一种新方法将用于调查离子和水运输 在睫状上皮细胞中。 来自 色素的兔子将被隔离，放置在专门设计的 腔室，并迅速灌注生理铃声。 定量光学显微镜将用于测量变化 在位于边缘的非色素细胞的体积中 睫状工艺，当灌注溶液更改为解决方案时 不同的渗透压，成分或温度。 一个 体积测量与细胞内离子的组合 活动测量将用于估计水 非色素上皮细胞的渗透性和 负责数量的主要无机离子的运输 调节和幽默的形成。 离子的本质 通过使用，将进一步阐明运输机制 已知的上皮运输抑制剂。 最后，我们会的 研究对非色素细胞的影响 用于治疗青光眼治疗的涉及剂。