ION AND WATER TRANSPORT IN CILIARY EPITHELIUM

睫状上皮中的离子和水传输

• 批准号: 3263735
• 负责人: NASSER A FARAHBAKHSH
• 金额: $ 13.54万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-09-30 至 1993-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3263735
• 关键词: acetazolamide; carbonic anhydrase inhibitors; cell morphology; electrophysiology; epithelium; eye pharmacology; fluorescence spectrometry; intraocular fluid; ion transport; laboratory rabbit; light microscopy; membrane permeability; secretion; uvea ciliary body

The long-term objective of this project is to identify the transporting mechanisms present in the ciliary body epithelial cells, and to elucidate their role, mode of action and possible interactions as relates to the secretion of aqueous humor, and regulation of cellular volume and pH. Quantitative light microscopy, fluorescence spectrophotometry, and intracellular recording will be combined to explore these mechanisms. Quantitative light microscopy will be used to measure the changes in the volume of the ciliary body epithelial cells when the tissue is exposed to changes in composition or osmolality of the surrounding medium. Fluorescence ratio microscopy will be utilized to measure changes in the intracellular activities of ions, including H+, that may underlie the volume changes, in order to identify respective ion transporters. Intracellular recordings, and the use of membrane ionophores and specific transport inhibitors will complement these studies by allowing us to decide between alternative candidates.

该项目的长期目标是确定运输 睫状体上皮细胞中存在的机制，并阐明 他们的作用，行动方式以及可能与 水性幽默的分泌以及细胞体积和pH的调节。 定量光学显微镜，荧光分光光度法和 细胞内记录将合并以探索这些机制。 定量光学显微镜将用于测量 当组织暴露于组织时，睫状体上皮细胞的体积 周围介质的组成或渗透率的变化。 荧光比显微镜将用于测量 离子的细胞内活性，包括H+，可能是基础的 体积变化，以识别各自的离子转运蛋白。 细胞内记录以及膜离子载体和特定的使用 运输抑制剂将通过允许我们决定来补充这些研究 在替代候选人之间。