RETINAL DEVELOPMENT: DENDRITIC GROWTH; REGENERATION

视网膜发育：树突生长；

• 批准号: 3263932
• 负责人: Peter F Hitchcock
• 金额: $ 19.23万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-05-01 至 1995-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3263932
• 关键词: afferent nerve; amacrine cells; autoradiography; biomarker; dendrites; electron microscopy; eye regeneration; fresh water environment; goldfish; growth cones; immunocytochemistry; interneurons; light microscopy; neural information processing; neuroanatomy; neurogenesis; retina; synapses

The long term objectives of the work proposed here are to contribute to our understanding of the mechanisms that underlie the development of the eye and brain. The immediate aims of this proposal are: 1) describe the contacts made by the dendritic growth cones of ganglion cells within the nascent inner plexiform layer; 2) determine whether or not the temporal differences in the electrical activity of afferent synapses is a guidance cue for dendritic growth 3) characterize the degree to which locally regenerated retina resembles the surrounding, undamaged retina; and 4) experimentally test whether or not the undamaged retina serves as a template upon which a local patch of retina is regenerated. These aims will be achieved by using electron microscopy, light microscopy, intracellular injections of individual retinal neurons with markers for light and electron microscopy, and immunocytochemistry. It is expected that the studies described herein will provide information that will eventually be useful for devising strategies for treating diseases of, or damage to the central nervous system.

这里提出的工作的长期目标是为我们的 理解眼睛发展的机制 和大脑。 该提议的直接目的是：1）描述 神经节细胞的树突生长锥在 新生的内丛状层； 2）确定是否时间 传入突触的电活动的差异是指导 树突生长的提示3）表征了局部的程度 再生视网膜类似于周围未损坏的视网膜；和4） 实验测试未损坏的视网膜是否充当 将当地视网膜斑块再生的模板。 这些目标 将通过使用电子显微镜，光学显微镜来实现 对单个视网膜神经元的细胞内注射标记 光和电子显微镜以及免疫细胞化学。 预计 本文所述的研究将提供信息 最终对于制定治疗疾病的策略或 中枢神经系统的损害。