STRUCTURAL NEUROCHEMISTRY OF RETINAL CIRCUITS

视网膜回路的结构神经化学

• 批准号: 2158463
• 负责人: ROBERT E MARC
• 金额: $ 17.05万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-03-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2158463
• 关键词: acetylcholine; alternatives to animals in research; amacrine cells; aspartate; autoradiography; dopamine; electron microscopy; electrophysiology; gamma aminobutyrate; glutamates; glycine; goldfish; histochemistry /cytochemistry; immunochemistry; immunocytochemistry; microscopy; neuroanatomy; neurochemistry; neurophysiology; neurotransmitter transport; neurotransmitters; psychophysics; radionuclide double label; retina; retinal bipolar neuron; retinal ganglion; serotonin; synapses; taurine; visual feedback

Neurotransmitter-specific synaptologies of vertebrate retinal neurons will be investigated in three program modules intended to describe retinal microcircuits with greater fidelity and provide an enhanced basis for comparing our knowledge of ectotherm visual processing with mammalian, especially human, visual performance. Module I: DOUBLE LABEL EXPERIMENTS (goldfish). Serial section electron microscope autoradiography of neurons selectively labeled by high affinity (3H) neurotransmitter uptake will be combined with: (A) Horseradish peroxidase histochemistry of ganglion cells filled by retrograde transport; (B) Gold-toning of Golgi-impregnated retinal neurons; or (C) Immunocytochemistry for putative peptide or conventional neurotransmitter containing neurons. These preparations will document the incidence of synaptic connections among GABAergic, glycinergic, serotoninergic, dopaminergic, putative taurinergic, putative peptidergic and morphologically characterized cells. The somatic and dendritic structures of neurochemically classified cells may be revealed by autoradiographic co-localization of (3H) neurotransmitter uptake in Golgi-impregnated cells. Module II: STRUCTURAL NEUROCHEMISTRY OF SALAMANDER RETINAS (mudpuppy and tiger salamander). Recognizing the continuing importance of urodele amphibians as physiological/pharmalogical models of visual processing, it has become critical to provide fundamental data on the types, distributions and connections of neurochemically defined retinal neurons. The urodele retina will be studied by serial section electron microscope autoradiography of (3H) GABA, glycine, taurine, serotonin and dopamine accumulating cells; immunocytochemistry will also be performed for some peptides, conventional neurotransmitters and their synthetic enzymes. Module III: EVALUATION OF CONVENTIONAL NEUROTRANSMITTERS IN AMACRINE AND BIPOLAR CELLS (goldfish, salamander, cat). Less thoroughly understood neurotransmitter systems (taurine, glycine, acetylcholine and acidic amino acids) will be evaluated through biochemical, autoradiographic, immunocytochemical or cytochemical means.

脊椎动物视网膜神经元的神经递质特异性突触 在三个旨在描述视网膜的程序模块中进行调查 微电路具有更大的保真度，并为 将我们对ectothotherm视觉处理的了解与哺乳动物的知识， 特别是人类的视觉表演。 模块I：双标签实验（金鱼）。 串行部分电子 神经元的显微镜放射自显影，由高亲和力选择性标记 （3H）神经递质的摄取将与：（a）辣根 逆行转运填充的神经节细胞的过氧化物酶组织化学； （b）高尔基体浸渍的视网膜神经元的金色；或（c） 假定肽或常规神经递质的免疫细胞化学 含有神经元。 这些准备工作将记录 GABA能，糖激素，5-羟色胺能之间的突触连接， 多巴胺能，推定的牛磺酸，推定的肽能和 形态学特征的细胞。 体细胞和树突结构 神经化学分类的细胞可以通过放射自显影揭示 （3H）神经递质摄取在高尔基体浸渍细胞中的共定位。 模块II：Salamander视网膜的结构神经化学（Mudpuppy和 老虎sal）。 认识到Urodele的持续重要性 两栖动物是视觉处理的生理/药物模型 对于提供有关类型，分布的基本数据至关重要 以及神经定义的视网膜神经元的连接。 urodele 视网膜将通过串行部分电子显微镜研究 （3H）GABA，甘氨酸，牛磺酸，5-羟色胺和多巴胺的放射自显影 积累细胞；某些人也将进行免疫细胞化学化学 肽，常规神经递质及其合成酶。 模块III：评估无氨氨意剂中的常规神经递质和 双极细胞（金鱼，sal，猫）。 不太了解 神经递质系统（牛磺酸，甘氨酸，乙酰胆碱和酸性氨基 酸）将通过生化，放射自显影术评估 免疫细胞化学或细胞化学方法。