COOPERATIVE CATARACT RESEARCH GROUP

白内障合作研究小组

• 批准号: 3259672
• 负责人: SIDNEY LERMAN
• 金额: $ 2.13万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-05-01 至 1989-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3259672
• 关键词: biomedical facility; cooperative study; densitometry; disease /disorder classification; eye bank /preservation; eye disorder diagnosis; information systems; lens proteins; noninvasive diagnosis; photography; spectrometry; ultraviolet radiation

Our long range goal is to delineate specific aspects of lens aging and cataractogenesis at the molecular level, particularly the photobiologic effects due to longwave ultraviolet (UVA) radiation. As well as utilizing our spectroscopic analysis (ultraviolet (UV) absorption and transmission, fluorescence, phosphorescence and EPR spectroscopy), we have expanded our approach to include nuclear magnetic resonance (NMR) spectroscopy to study the normal lens and the effects of UV radiation on human and animal lenses and protein extracts. Our current aim is to further expand this approach in order to develop surface scanning NMR capable of monitoring specific parameters (e.g., organophosphate metabolism) in the living animal lens as delineated by our in vitro studies (on human and animal lenses). The foregoing in vitro studies will enable us to determine age related changes with respect to biophysical aspects of lens transparency in aging and senile cataractogenesis. Our photobiologic studies have enabled us to develop an in vivo method (UV slit lamp densitography) to screen for and measure direct and photosensitized lens damage. The projected surface scanning NMR studies could result in the development of a second in vivo screening method. The in vivo UV-visible slit lamp densitography will be performed on patients prior to cataract surgery; the intact lens is then photographed (with the CCRG method) immediately after extraction and then subjected to the optical and NMR spectroscopic analyses. These studies will permit a direct correlation between an in vitro and in vivo cataract classification system as well as determining objective spectroscopic parameters associated with various types of human cataract. Since aging is accompanied by measureable changes in lens transparency and fluorescence, the foregoing approach on fresh whole eye bank eyes (UV-visible slit lamp densitography) followed by a second series of UV-visible photographs and CCRG photographs on the excised lens will provide significant data on the processes associated with the documented age realted changes in lens fluorescence, phosphorescence and transparency. These data will be correlated with directs spectroscopic analyses on the intact lens and should provide new information on the age related changes resulting in the development of localized scattering elements (opacities) which eventually become manifest in the vast majority of aging "normal" lenses.

我们的远距离目标是描述镜头衰老的特定方面 分子水平的白内生生成，尤其是光生物学 由于长波紫外线（UVA）辐射引起的影响。 以及利用 我们的光谱分析（紫外线（UV）吸收和传播， 荧光，磷光和EPR光谱），我们已经扩展了我们的 包括核磁共振（NMR）光谱的方法 正常晶状体和紫外线辐射对人类和动物镜头的影响 和蛋白质提取物。 我们目前的目标是进一步扩展这种方法 为了开发表面扫描NMR，能够监视特定 活动物镜头中的参数（例如有机磷酸盐代谢） 通过我们的体外研究（关于人和动物镜头）的描述。 上述体外研究将使我们能够确定与年龄相关的 在衰老中透明度的生物物理方面的变化 和白内垂质发生。 我们的光生物学研究使我们能够 开发一种体内方法（紫外缝灯光密摄影）以筛选和 测量直接和光敏的镜头损伤。 投影表面 扫描NMR研究可能导致第二个体内发展 筛选方法。 体内紫外可见的缝隙光密摄影将是 在白内障手术前对患者进行；那是完整的镜头 提取后立即拍摄（使用CCRG方法），然后 进行光学和NMR光谱分析。 这些研究 将允许体外和体内白内障之间的直接相关 分类系统以及确定客观光谱 与各种类型的人白内障相关的参数。 因为衰老是 伴随着透镜透明度和荧光的可测量变化， 新鲜的全眼睛眼睛的上述方法（紫外可见的缝灯灯 光密摄影），然后是第二个紫外可见的照片， 切除镜头上的CCRG照片将提供有关 与记录的年龄实现镜头变化相关的过程 荧光，磷光和透明度。 这些数据将是 与完整晶状体的直接光谱分析相关 应提供有关年龄相关变化的新信息，导致 最终的局部散射元素（不透明）的发展 在绝大多数衰老的“正常”镜片中变得明显。